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The pancreatic β-cell recognition of insulin secretagogues. Comparisons of glucose with glyceraldehyde isomers and dihydroxyacetone

Hellman, Bo ; Idahl, Lars Åke ; Lernmark, Åke LU ; Sehlin, Janove and Täljedal, Inge Bert (1974) In Archives of Biochemistry and Biophysics 162(2). p.448-457
Abstract

d-Glyceraldehyde stimulated the release of insulin from pancreatic islets of Umeå- ob ob-mice whether or not glucose was present in the medium. Like the action of glucose, that of d-glyceraldehyde was biphasic in time, exhibited a sigmoidal dose-response relationship, was potentiated by theophylline, arginine, iodoacetamide, or l-glyceraldehyde, and was inhibited by epinephrine, 2,4-dinitrophenol, or Ca2+ deficiency. Half-maximum and maximum stimulations were produced by about 3 mm and 10 mm d-glyceraldehyde. Positive interactions were observed between 5 mm d-glyceraldehyde and 5 mm glucose and between 10 mm d-glyceraldehyde and 10 mm leucine. Mannoheptulose (10 mm) or glucosamine (10 mm) did not inhibit but potentiated the... (More)

d-Glyceraldehyde stimulated the release of insulin from pancreatic islets of Umeå- ob ob-mice whether or not glucose was present in the medium. Like the action of glucose, that of d-glyceraldehyde was biphasic in time, exhibited a sigmoidal dose-response relationship, was potentiated by theophylline, arginine, iodoacetamide, or l-glyceraldehyde, and was inhibited by epinephrine, 2,4-dinitrophenol, or Ca2+ deficiency. Half-maximum and maximum stimulations were produced by about 3 mm and 10 mm d-glyceraldehyde. Positive interactions were observed between 5 mm d-glyceraldehyde and 5 mm glucose and between 10 mm d-glyceraldehyde and 10 mm leucine. Mannoheptulose (10 mm) or glucosamine (10 mm) did not inhibit but potentiated the effect of 10 mm d-glyceraldehyde. Dihydroxyacetone (2.5-20 mm) also initiated insulin release in the absence of glucose. On the other hand, 5-10 mm l-glyceraldehyde did not initiate secretion but potentiated the effects of 5 mm glucose or 5 mm d-glyceraldehyde. d-Glyceraldehyde or dihydroxyacetone reduced the production of 14CO2 from d-[U-14C]glucose; l-glyceraldehyde had a smaller and statistically insignificant effect. The results suggest that by being phosphorylated and entering glycolysis in the β-cells, d-glyceraldehyde and dihydroxyacetone act as functional analogues of glucose as secretory stimulus. Initiation of insulin release by glucose, d-glyceraldehyde, or dihydroxyacetone may thus depend on the production of a metabolic signal at or below the triose phosphate level.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Archives of Biochemistry and Biophysics
volume
162
issue
2
pages
10 pages
publisher
Academic Press
external identifiers
  • pmid:4210076
  • scopus:0016192841
ISSN
0003-9861
DOI
10.1016/0003-9861(74)90204-5
language
English
LU publication?
no
id
1975c277-73d2-46b2-9a9b-e470d0936af6
date added to LUP
2019-09-18 12:17:46
date last changed
2020-01-13 02:23:19
@article{1975c277-73d2-46b2-9a9b-e470d0936af6,
  abstract     = {<p>d-Glyceraldehyde stimulated the release of insulin from pancreatic islets of Umeå- ob ob-mice whether or not glucose was present in the medium. Like the action of glucose, that of d-glyceraldehyde was biphasic in time, exhibited a sigmoidal dose-response relationship, was potentiated by theophylline, arginine, iodoacetamide, or l-glyceraldehyde, and was inhibited by epinephrine, 2,4-dinitrophenol, or Ca<sup>2+</sup> deficiency. Half-maximum and maximum stimulations were produced by about 3 mm and 10 mm d-glyceraldehyde. Positive interactions were observed between 5 mm d-glyceraldehyde and 5 mm glucose and between 10 mm d-glyceraldehyde and 10 mm leucine. Mannoheptulose (10 mm) or glucosamine (10 mm) did not inhibit but potentiated the effect of 10 mm d-glyceraldehyde. Dihydroxyacetone (2.5-20 mm) also initiated insulin release in the absence of glucose. On the other hand, 5-10 mm l-glyceraldehyde did not initiate secretion but potentiated the effects of 5 mm glucose or 5 mm d-glyceraldehyde. d-Glyceraldehyde or dihydroxyacetone reduced the production of <sup>14</sup>CO<sub>2</sub> from d-[U-<sup>14</sup>C]glucose; l-glyceraldehyde had a smaller and statistically insignificant effect. The results suggest that by being phosphorylated and entering glycolysis in the β-cells, d-glyceraldehyde and dihydroxyacetone act as functional analogues of glucose as secretory stimulus. Initiation of insulin release by glucose, d-glyceraldehyde, or dihydroxyacetone may thus depend on the production of a metabolic signal at or below the triose phosphate level.</p>},
  author       = {Hellman, Bo and Idahl, Lars Åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert},
  issn         = {0003-9861},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {448--457},
  publisher    = {Academic Press},
  series       = {Archives of Biochemistry and Biophysics},
  title        = {The pancreatic β-cell recognition of insulin secretagogues. Comparisons of glucose with glyceraldehyde isomers and dihydroxyacetone},
  url          = {http://dx.doi.org/10.1016/0003-9861(74)90204-5},
  doi          = {10.1016/0003-9861(74)90204-5},
  volume       = {162},
  year         = {1974},
}