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Endotoxins in Environmental and Clinical Samples Assessed by GC-Tandem MS

Szponar, Bogumila LU (2010) NATO Advanced Research Workshop on Detection of Biological Agents for the Prevention of Bioterrorism In Detection of Biological Agents for the Prevention of Bioterrorism p.245-265
Abstract
Bacteria appeared on the Earth millions years before us and human evolution was triggered by the constant presence of pathogenic and symbiotic microorganisms in our surroundings. Interplay occurred between higher organism and microbial consortia residing in the host organs and on the epithelial surfaces; another natural space of bacteria human interaction is the indoor environment where we spend the majority of our lifetime. Indoor microbial exposure affects our well-being and can result in respiratory symptoms, such as allergies and asthma, since both dead and live microorganisms and their cell constituents, including lipopolysaccharides (LPS, endotoxins), interact with our immune system. Thus, there is a demand for robust tools for... (More)
Bacteria appeared on the Earth millions years before us and human evolution was triggered by the constant presence of pathogenic and symbiotic microorganisms in our surroundings. Interplay occurred between higher organism and microbial consortia residing in the host organs and on the epithelial surfaces; another natural space of bacteria human interaction is the indoor environment where we spend the majority of our lifetime. Indoor microbial exposure affects our well-being and can result in respiratory symptoms, such as allergies and asthma, since both dead and live microorganisms and their cell constituents, including lipopolysaccharides (LPS, endotoxins), interact with our immune system. Thus, there is a demand for robust tools for qualitative and quantitative determination of the microbial communities that we are exposed to. This work described the reproducible approach of the Gram-negative bacteria and endotoxins assessment by their specific chemical markers, 3-hydroxy fatty acids. Gas chromatography-tandem mass spectrometry proved to be an excellent means for specific and selective detection of bacteria/endotoxin markers in the complex matrices like indoor bioaerosol and clinical samples: blood, saliva or feces. Using this method, epidemiological studies were conducted in the field of indoor air quality research, as well as in clinical investigations when bacterial consortia were involved: in Crohn's disease, periodontitis, and newborn gut microbial colonisation in association with allergy development. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
Detection of Biological Agents for the Prevention of Bioterrorism
pages
245 - 265
publisher
Springer
conference name
NATO Advanced Research Workshop on Detection of Biological Agents for the Prevention of Bioterrorism
external identifiers
  • wos:000290640100016
ISSN
1874-6489
DOI
10.1007/978-90-481-9815-3_16
language
English
LU publication?
yes
id
5d1d1ba5-ed71-43c9-a07d-2237e5fcb072 (old id 1984589)
date added to LUP
2011-07-08 16:07:13
date last changed
2018-05-29 10:28:50
@inproceedings{5d1d1ba5-ed71-43c9-a07d-2237e5fcb072,
  abstract     = {Bacteria appeared on the Earth millions years before us and human evolution was triggered by the constant presence of pathogenic and symbiotic microorganisms in our surroundings. Interplay occurred between higher organism and microbial consortia residing in the host organs and on the epithelial surfaces; another natural space of bacteria human interaction is the indoor environment where we spend the majority of our lifetime. Indoor microbial exposure affects our well-being and can result in respiratory symptoms, such as allergies and asthma, since both dead and live microorganisms and their cell constituents, including lipopolysaccharides (LPS, endotoxins), interact with our immune system. Thus, there is a demand for robust tools for qualitative and quantitative determination of the microbial communities that we are exposed to. This work described the reproducible approach of the Gram-negative bacteria and endotoxins assessment by their specific chemical markers, 3-hydroxy fatty acids. Gas chromatography-tandem mass spectrometry proved to be an excellent means for specific and selective detection of bacteria/endotoxin markers in the complex matrices like indoor bioaerosol and clinical samples: blood, saliva or feces. Using this method, epidemiological studies were conducted in the field of indoor air quality research, as well as in clinical investigations when bacterial consortia were involved: in Crohn's disease, periodontitis, and newborn gut microbial colonisation in association with allergy development.},
  author       = {Szponar, Bogumila},
  booktitle    = {Detection of Biological Agents for the Prevention of Bioterrorism},
  issn         = {1874-6489},
  language     = {eng},
  pages        = {245--265},
  publisher    = {Springer},
  title        = {Endotoxins in Environmental and Clinical Samples Assessed by GC-Tandem MS},
  url          = {http://dx.doi.org/10.1007/978-90-481-9815-3_16},
  year         = {2010},
}