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Effects of ionic substances in bleaching filtrates and of lignosulfonates on the activity of oxalate oxidase from barley

Winestrand, Sandra; Larsson, Simona; Cassland, Pierre LU ; Nilvebrant, Nils-Olof and Jonsson, Leif J. (2011) In Engineering in Life Sciences 11(3). p.245-252
Abstract
The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of... (More)
The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of 3.8, only 2-16% of the activity of oxalate oxidase remained. The results show the effects of anionic and cationic substances in bleaching filtrates on oxalate oxidase and indicate that there is an interaction between the enzyme, which has a positive net charge at pH 3.8, and the polymeric anionic lignosulfonates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lignosulfonates, Oxalate oxidase, Pulp and paper
in
Engineering in Life Sciences
volume
11
issue
3
pages
245 - 252
publisher
Wiley-Blackwell
external identifiers
  • wos:000291556600003
  • scopus:79958839952
ISSN
1618-2863
DOI
10.1002/elsc.201000156
language
English
LU publication?
yes
id
a1e3b7a0-6133-4018-b017-7a10c2d873c9 (old id 1984971)
date added to LUP
2011-06-29 11:24:27
date last changed
2017-01-01 03:28:51
@article{a1e3b7a0-6133-4018-b017-7a10c2d873c9,
  abstract     = {The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of 3.8, only 2-16% of the activity of oxalate oxidase remained. The results show the effects of anionic and cationic substances in bleaching filtrates on oxalate oxidase and indicate that there is an interaction between the enzyme, which has a positive net charge at pH 3.8, and the polymeric anionic lignosulfonates.},
  author       = {Winestrand, Sandra and Larsson, Simona and Cassland, Pierre and Nilvebrant, Nils-Olof and Jonsson, Leif J.},
  issn         = {1618-2863},
  keyword      = {Lignosulfonates,Oxalate oxidase,Pulp and paper},
  language     = {eng},
  number       = {3},
  pages        = {245--252},
  publisher    = {Wiley-Blackwell},
  series       = {Engineering in Life Sciences},
  title        = {Effects of ionic substances in bleaching filtrates and of lignosulfonates on the activity of oxalate oxidase from barley},
  url          = {http://dx.doi.org/10.1002/elsc.201000156},
  volume       = {11},
  year         = {2011},
}