Effects of ionic substances in bleaching filtrates and of lignosulfonates on the activity of oxalate oxidase from barley
(2011) In Engineering in Life Sciences 11(3). p.245-252- Abstract
- The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of... (More)
- The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of 3.8, only 2-16% of the activity of oxalate oxidase remained. The results show the effects of anionic and cationic substances in bleaching filtrates on oxalate oxidase and indicate that there is an interaction between the enzyme, which has a positive net charge at pH 3.8, and the polymeric anionic lignosulfonates. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1984971
- author
- Winestrand, Sandra ; Larsson, Simona ; Cassland, Pierre LU ; Nilvebrant, Nils-Olof and Jonsson, Leif J.
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lignosulfonates, Oxalate oxidase, Pulp and paper
- in
- Engineering in Life Sciences
- volume
- 11
- issue
- 3
- pages
- 245 - 252
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000291556600003
- scopus:79958839952
- ISSN
- 1618-2863
- DOI
- 10.1002/elsc.201000156
- language
- English
- LU publication?
- yes
- id
- a1e3b7a0-6133-4018-b017-7a10c2d873c9 (old id 1984971)
- date added to LUP
- 2016-04-01 10:19:25
- date last changed
- 2022-01-25 22:07:24
@article{a1e3b7a0-6133-4018-b017-7a10c2d873c9,
abstract = {{The effects of ionic substances in seven industrial filtrates from kraft pulping, mechanical pulping, and sulfite pulping on the activity of oxalate oxidase from barley were investigated by pre-treatment of the filtrates with ion-exchange resins prior to enzymatic degradation of the oxalic acid in the filtrates. The pre-treatment resulted in increased oxalic acid degradation rates in all filtrates, except for one that was obtained from sulfite pulping. The possibility that lignosulfonates, which were present in the filtrate from sulfite pulping, could affect oxalate oxidase was investigated in a separate set of experiments involving four different preparations of lignosulfonates. At a lignosulfonate concentration of 50 mg/mL and a pH of 3.8, only 2-16% of the activity of oxalate oxidase remained. The results show the effects of anionic and cationic substances in bleaching filtrates on oxalate oxidase and indicate that there is an interaction between the enzyme, which has a positive net charge at pH 3.8, and the polymeric anionic lignosulfonates.}},
author = {{Winestrand, Sandra and Larsson, Simona and Cassland, Pierre and Nilvebrant, Nils-Olof and Jonsson, Leif J.}},
issn = {{1618-2863}},
keywords = {{Lignosulfonates; Oxalate oxidase; Pulp and paper}},
language = {{eng}},
number = {{3}},
pages = {{245--252}},
publisher = {{Wiley-Blackwell}},
series = {{Engineering in Life Sciences}},
title = {{Effects of ionic substances in bleaching filtrates and of lignosulfonates on the activity of oxalate oxidase from barley}},
url = {{http://dx.doi.org/10.1002/elsc.201000156}},
doi = {{10.1002/elsc.201000156}},
volume = {{11}},
year = {{2011}},
}