Advanced

Evaluation of Steam-Treated Giant Bamboo for Production of Fermentable Sugars

Garcia-Aparicio, Maria; Parawira, Wilson; Van Rensburg, Eugene; Diedericks, Danie; Galbe, Mats LU ; Roslander, Christian LU ; Zacchi, Guido LU and Goergens, Johann (2011) In Biotechnology Progress 27(3). p.641-649
Abstract
Giant bamboo plantations are currently being established in the Southern Africa region and can be considered as potential lignocellulosic feedstock for the production of second generation bioethanol. In this study, giant bamboo internodal material was subjected to sulphur dioxide (SO2) impregnated steam pretreatment prior to enzymatic hydrolysis. The effect of temperature, residence time, and acidity on the overall sugar recovery and byproduct formation was studied using response surface response technology according to a central composite experimental design (CCD) at a fixed SO2 concentration of 2.5% (w/w liquid) after impregnation. The results showed that pretreatment conditions with combined severity factor (CSF) values and enzyme... (More)
Giant bamboo plantations are currently being established in the Southern Africa region and can be considered as potential lignocellulosic feedstock for the production of second generation bioethanol. In this study, giant bamboo internodal material was subjected to sulphur dioxide (SO2) impregnated steam pretreatment prior to enzymatic hydrolysis. The effect of temperature, residence time, and acidity on the overall sugar recovery and byproduct formation was studied using response surface response technology according to a central composite experimental design (CCD) at a fixed SO2 concentration of 2.5% (w/w liquid) after impregnation. The results showed that pretreatment conditions with combined severity factor (CSF) values and enzyme dosages greater than 1.72 and 30 FPU/g water insoluble solid, respectively, were required to obtain an efficient glucan digestibility and a good overall glucose recovery. Up to 81.2% of the sugar in the raw material was recovered for a CSF of 2.25. However, considering overall sugar yield and byproducts concentration, the pretreated material obtained with a CSF of 1.62 can be considered as the most appropriate for SSF experiments using a xylose-utilizing yeast. At these conditions, it could be possible to obtain up to 247 L of ethanol per dry ton of giant bamboo considering hexose and pentose sugars fermentation. This amount could be increased up to 292 L of ethanol per dry ton of giant bamboo with the maximum sugar yield obtained (CSF 2.25) if the microorganism possesses robust fermentative characteristics as well as a high resistance to pretreatment by-products. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 641-649, 2011 (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bamboo, SO2-catalyzed steam pretreatment, combined severity factor, enzymatic hydrolysis, bioethanol
in
Biotechnology Progress
volume
27
issue
3
pages
641 - 649
publisher
The American Chemical Society
external identifiers
  • wos:000291544000005
  • scopus:79958254792
ISSN
1520-6033
DOI
10.1002/btpr.580
language
English
LU publication?
yes
id
6c717565-4f88-40f8-9685-3562ad5d61e6 (old id 1985062)
date added to LUP
2011-07-08 09:47:11
date last changed
2017-06-18 04:15:30
@article{6c717565-4f88-40f8-9685-3562ad5d61e6,
  abstract     = {Giant bamboo plantations are currently being established in the Southern Africa region and can be considered as potential lignocellulosic feedstock for the production of second generation bioethanol. In this study, giant bamboo internodal material was subjected to sulphur dioxide (SO2) impregnated steam pretreatment prior to enzymatic hydrolysis. The effect of temperature, residence time, and acidity on the overall sugar recovery and byproduct formation was studied using response surface response technology according to a central composite experimental design (CCD) at a fixed SO2 concentration of 2.5% (w/w liquid) after impregnation. The results showed that pretreatment conditions with combined severity factor (CSF) values and enzyme dosages greater than 1.72 and 30 FPU/g water insoluble solid, respectively, were required to obtain an efficient glucan digestibility and a good overall glucose recovery. Up to 81.2% of the sugar in the raw material was recovered for a CSF of 2.25. However, considering overall sugar yield and byproducts concentration, the pretreated material obtained with a CSF of 1.62 can be considered as the most appropriate for SSF experiments using a xylose-utilizing yeast. At these conditions, it could be possible to obtain up to 247 L of ethanol per dry ton of giant bamboo considering hexose and pentose sugars fermentation. This amount could be increased up to 292 L of ethanol per dry ton of giant bamboo with the maximum sugar yield obtained (CSF 2.25) if the microorganism possesses robust fermentative characteristics as well as a high resistance to pretreatment by-products. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 641-649, 2011},
  author       = {Garcia-Aparicio, Maria and Parawira, Wilson and Van Rensburg, Eugene and Diedericks, Danie and Galbe, Mats and Roslander, Christian and Zacchi, Guido and Goergens, Johann},
  issn         = {1520-6033},
  keyword      = {bamboo,SO2-catalyzed steam pretreatment,combined severity factor,enzymatic hydrolysis,bioethanol},
  language     = {eng},
  number       = {3},
  pages        = {641--649},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Evaluation of Steam-Treated Giant Bamboo for Production of Fermentable Sugars},
  url          = {http://dx.doi.org/10.1002/btpr.580},
  volume       = {27},
  year         = {2011},
}