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CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization

Tormin, Ariane LU ; Li, Ou LU ; Brune, Jan Claas LU ; Walsh, Stuart LU ; Schütz, Birgit LU ; Ehinger, Mats LU ; Ditzel, Nicholas; Kassem, Moustapha and Scheding, Stefan LU (2011) In Blood 117(19). p.5067-5077
Abstract
Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin(-)/CD271(+)/CD45(-)/CD146(+) stem-cell fraction, but also in lin(-)/CD271(+)/CD45(-)/CD146(-/low) cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly,... (More)
Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin(-)/CD271(+)/CD45(-)/CD146(+) stem-cell fraction, but also in lin(-)/CD271(+)/CD45(-)/CD146(-/low) cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly, CD146 was up-regulated in normoxia and down-regulated in hypoxia. This was correlated with in situ localization differences, with CD146 coexpressing reticular cells located in perivascular regions, whereas bone-lining MSCs expressed CD271 alone. In both regions, CD34(+) hematopoietic stem/progenitor cells were located in close proximity to MSCs. These novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic BM-MSC populations, which may be useful for the study of the hematopoietic environment. (Blood. 2011; 117(19): 5067-5077) (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
117
issue
19
pages
5067 - 5077
publisher
American Society of Hematology
external identifiers
  • wos:000290528900013
  • pmid:21415267
  • scopus:79955944297
ISSN
1528-0020
DOI
10.1182/blood-2010-08-304287
language
English
LU publication?
yes
id
b11e9974-315f-4375-b60a-88be4cc4676c (old id 1987037)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21415267?dopt=Abstract
date added to LUP
2011-07-01 09:22:22
date last changed
2017-10-01 03:26:25
@article{b11e9974-315f-4375-b60a-88be4cc4676c,
  abstract     = {Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin(-)/CD271(+)/CD45(-)/CD146(+) stem-cell fraction, but also in lin(-)/CD271(+)/CD45(-)/CD146(-/low) cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly, CD146 was up-regulated in normoxia and down-regulated in hypoxia. This was correlated with in situ localization differences, with CD146 coexpressing reticular cells located in perivascular regions, whereas bone-lining MSCs expressed CD271 alone. In both regions, CD34(+) hematopoietic stem/progenitor cells were located in close proximity to MSCs. These novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic BM-MSC populations, which may be useful for the study of the hematopoietic environment. (Blood. 2011; 117(19): 5067-5077)},
  author       = {Tormin, Ariane and Li, Ou and Brune, Jan Claas and Walsh, Stuart and Schütz, Birgit and Ehinger, Mats and Ditzel, Nicholas and Kassem, Moustapha and Scheding, Stefan},
  issn         = {1528-0020},
  language     = {eng},
  number       = {19},
  pages        = {5067--5077},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization},
  url          = {http://dx.doi.org/10.1182/blood-2010-08-304287},
  volume       = {117},
  year         = {2011},
}