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Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics

Popovic, Zoran; Knutsson, Per; Thaung, Jorgen; Owner-Petersen, Mette LU and Sjostrand, Johan (2011) In Investigative Ophthalmology & Visual Science 52(5). p.2649-2655
Abstract
PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument. METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius... (More)
PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument. METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius from the foveal center) and in the superior, inferior, temporal, and nasal quadrants within the two ROIs. RESULTS. Mean FAZ area was 0.302 +/- 0.100 mm(2), and mean capillary density (length/area) in the inner ROI was 38.0 +/- 4.0 mm(-1) and 36.4 +/- 4.0 mm(-1) in the outer ROI. The difference in ROI capillary density was not significant. There was no significant difference in quadrant capillary density within the two ROIs or between quadrants irrespective of ROI. CONCLUSIONS. The authors have demonstrated a technique for noninvasive imaging and semiautomated detection and analysis of foveal capillaries. In comparison with other studies, their method yielded lower capillary densities than histology but similar results to the current clinical gold standard, fluorescein angiography. The increased field of view of the DCAO instrument opens up new possibilities for high-resolution noninvasive clinical imaging of foveal capillaries. (Invest Ophthalmol Vis Sci. 2011;52:2649-2655) DOI:10.1167/iovs.10-6054 (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Investigative Ophthalmology & Visual Science
volume
52
issue
5
pages
2649 - 2655
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • wos:000290111000019
  • scopus:79956055500
ISSN
1552-5783
DOI
10.1167/iovs.10-6054
language
English
LU publication?
yes
id
1d47ff35-6c2b-4f03-be3b-6c54e607c134 (old id 1987821)
date added to LUP
2011-06-28 14:12:52
date last changed
2017-10-22 04:28:13
@article{1d47ff35-6c2b-4f03-be3b-6c54e607c134,
  abstract     = {PURPOSE. To demonstrate noninvasive imaging of human foveal capillary networks with a high-resolution, wide-field, dual-conjugate adaptive optics (DCAO) imaging instrument. METHODS. The foveal capillary networks of five healthy subjects with no previous history of ocular or neurologic disease or surgery were imaged with a novel high-resolution, wide-field DCAO instrument. The foveal avascular zone (FAZ) in each image was defined using a manual procedure. An automated algorithm based on publicly available and custom-written software was used to identify vessels and extract morphologic FAZ and vessel parameters. Capillary densities were calculated in two annular regions of interest (ROIs) outside the FAZ (500 mu m and 750 mu m outer radius from the foveal center) and in the superior, inferior, temporal, and nasal quadrants within the two ROIs. RESULTS. Mean FAZ area was 0.302 +/- 0.100 mm(2), and mean capillary density (length/area) in the inner ROI was 38.0 +/- 4.0 mm(-1) and 36.4 +/- 4.0 mm(-1) in the outer ROI. The difference in ROI capillary density was not significant. There was no significant difference in quadrant capillary density within the two ROIs or between quadrants irrespective of ROI. CONCLUSIONS. The authors have demonstrated a technique for noninvasive imaging and semiautomated detection and analysis of foveal capillaries. In comparison with other studies, their method yielded lower capillary densities than histology but similar results to the current clinical gold standard, fluorescein angiography. The increased field of view of the DCAO instrument opens up new possibilities for high-resolution noninvasive clinical imaging of foveal capillaries. (Invest Ophthalmol Vis Sci. 2011;52:2649-2655) DOI:10.1167/iovs.10-6054},
  author       = {Popovic, Zoran and Knutsson, Per and Thaung, Jorgen and Owner-Petersen, Mette and Sjostrand, Johan},
  issn         = {1552-5783},
  language     = {eng},
  number       = {5},
  pages        = {2649--2655},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology & Visual Science},
  title        = {Noninvasive Imaging of Human Foveal Capillary Network Using Dual-Conjugate Adaptive Optics},
  url          = {http://dx.doi.org/10.1167/iovs.10-6054},
  volume       = {52},
  year         = {2011},
}