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Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce a recombinant antimicrobial peptide

Park, Heung-Bok ; Pyo, Sang-Hyun LU ; Hong, Seung-Suh and Kim, Jin-Hyun (2001) In Biotechnology Letters 23(8). p.637-641
Abstract
Hydroxylamine was used to cleave the Asn-Gly peptide bond between the
fusion partner and the antimicrobial peptide of interest, a magainin
derivative (MSI-344). The efficiency of reaction depended on the
hydroxylamine concentration, denaturant, pH, and the fused protein
concentration. The optimal cleavage solution consisted of guanidine ⋅
HCl as the denaturant, pH 8.1, and 6.7 mg ml−1 of fused MSI-344. This optimized cleavage solution resulted in a high yield (∼95% ) of MSI-344 from a cultivation of E. coli. This result suggests potential applications for using hydroxylamine to cleave basic peptides produced from fusion proteins.
Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Contribution to journal
publication status
published
in
Biotechnology Letters
volume
23
issue
8
pages
637 - 641
publisher
Springer Science and Business Media B.V.
external identifiers
  • scopus:0035038213
ISSN
0141-5492
DOI
10.1023/A:1010358131124
language
English
LU publication?
no
id
1a053314-c510-4524-a6fb-4cb8f57659ca
date added to LUP
2025-09-05 17:38:31
date last changed
2025-09-16 12:34:15
@article{1a053314-c510-4524-a6fb-4cb8f57659ca,
  abstract     = {{Hydroxylamine was used to cleave the Asn-Gly peptide bond between the <br>
fusion partner and the antimicrobial peptide of interest, a magainin <br>
derivative (MSI-344). The efficiency of reaction depended on the <br>
hydroxylamine concentration, denaturant, pH, and the fused protein <br>
concentration. The optimal cleavage solution consisted of guanidine ⋅ <br>
HCl as the denaturant, pH 8.1, and 6.7 mg ml<sup>−1</sup> of fused MSI-344. This optimized cleavage solution resulted in a high yield (∼95% ) of MSI-344 from a cultivation of <i>E. coli</i>. This result suggests potential applications for using hydroxylamine to cleave basic peptides produced from fusion proteins.}},
  author       = {{Park, Heung-Bok and Pyo, Sang-Hyun and Hong, Seung-Suh and Kim, Jin-Hyun}},
  issn         = {{0141-5492}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{637--641}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce a recombinant antimicrobial peptide}},
  url          = {{http://dx.doi.org/10.1023/A:1010358131124}},
  doi          = {{10.1023/A:1010358131124}},
  volume       = {{23}},
  year         = {{2001}},
}