Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation
(1986) In Proc Natl Acad Sci U S A 83(18). p.20-6716- Abstract
- Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to... (More)
- Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965135
- author
- Lundwall, Åke LU ; Dackowski, W. ; Cohen, E. ; Shaffer, M. ; Mahr, A. ; Dahlback, B. ; Stenflo, J. and Wydro, R.
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Protein S, Humans, Glycoproteins/analysis/*genetics, Epidermal Growth Factor/analysis, DNA/*analysis/isolation & purification, Amino Acid Sequence, Base Sequence, RNA, Messenger/analysis, Research Support, Non-U.S. Gov't
- in
- Proc Natl Acad Sci U S A
- volume
- 83
- issue
- 18
- pages
- 20 - 6716
- external identifiers
-
- scopus:0010336865
- language
- English
- LU publication?
- yes
- additional info
- 18
- id
- 1a06e725-e06e-4573-97eb-3c51a29c7f54 (old id 3965135)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2944113
- date added to LUP
- 2016-04-04 14:18:27
- date last changed
- 2021-04-25 05:30:12
@article{1a06e725-e06e-4573-97eb-3c51a29c7f54,
abstract = {{Protein S is a cofactor of activated protein C; together they function as a regulator of blood coagulation. A human liver cDNA library constructed in bacteriophage lambda gt11 was screened with DNA fragments from a full-length bovine cDNA clone encoding protein S. Several cDNA clones were isolated and sequenced. The combined cDNA sequences encoded the mature protein and 15 residues of the leader sequence when compared to bovine protein S. Human protein S is a single-chain protein consisting of 635 amino acids with 82% homology to bovine protein S. After an NH2-terminal gamma-carboxyglutamic acid-containing region, there is a short region with thrombin-sensitive bond(s), followed by a region with four repeat sequences that are homologous to the precursor of mouse epidermal growth factor. In contrast to the other vitamin K-dependent plasma proteins, the COOH-terminal portion of human protein S does not show any resemblance to serine proteases.}},
author = {{Lundwall, Åke and Dackowski, W. and Cohen, E. and Shaffer, M. and Mahr, A. and Dahlback, B. and Stenflo, J. and Wydro, R.}},
keywords = {{Protein S; Humans; Glycoproteins/analysis/*genetics; Epidermal Growth Factor/analysis; DNA/*analysis/isolation & purification; Amino Acid Sequence; Base Sequence; RNA; Messenger/analysis; Research Support; Non-U.S. Gov't}},
language = {{eng}},
number = {{18}},
pages = {{20--6716}},
series = {{Proc Natl Acad Sci U S A}},
title = {{Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation}},
url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2944113}},
volume = {{83}},
year = {{1986}},
}