Ultrastructure of alpha-synuclein-positive aggregations in U373 astrocytoma and rat primary glial cells
(2002) In Neuroscience Letters 323(1). p.37-40- Abstract
- Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is... (More)
- Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is intensely alpha-synuclein-immunopositive and a predominantly filamentous halo around. Mainly filamentous structures at the border area between the halo and the core are alpha-synuclein-immunoreactive. We conclude that this in vitro model of alpha-synuclein-positive glial aggregations mimics the morphology of the abnormal glial inclusions described in neuroclegenerative disorders and could be a suitable model for studying their role in the pathogenesis of these diseases. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/340572
- author
- Stefanova, N ; Emgård-Mattson, Mia LU ; Klimaschewski, L ; Wenning, GK and Reindl, M
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glial cytoplasmic inclusions, ultrastructure, alpha-synuclein, glia, Lewy bodies, neurodegeneration
- in
- Neuroscience Letters
- volume
- 323
- issue
- 1
- pages
- 37 - 40
- publisher
- Elsevier
- external identifiers
-
- pmid:11911985
- wos:000174853100010
- scopus:0037134107
- ISSN
- 0304-3940
- DOI
- 10.1016/S0304-3940(02)00117-9
- language
- English
- LU publication?
- yes
- id
- 1a2bb052-c2b3-47b3-b34d-70f700c2f4a9 (old id 340572)
- date added to LUP
- 2016-04-01 12:31:27
- date last changed
- 2022-03-29 02:02:49
@article{1a2bb052-c2b3-47b3-b34d-70f700c2f4a9,
abstract = {{Abnormal alpha-synuclein-positive glial cytoplasmic inclusions are found in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. We have recently developed an in vitro model of alpha-synuclein-immunoreactive aggregations in U373 astrocytoma cells. We have additionally overexpressed wild-type and a C-terminally truncated form of alpha-synuclein in primary rat glial cells. Astrocytes and oligodendrocytes were found to form alpha-synuclein-positive aggregations in vitro perinuclearly or in the processes of the cells. The morphological studies presented here demonstrate that the aggregations we have observed in vitro are not limited by a membrane but have unclear borders. They have an amorphous dense core that is intensely alpha-synuclein-immunopositive and a predominantly filamentous halo around. Mainly filamentous structures at the border area between the halo and the core are alpha-synuclein-immunoreactive. We conclude that this in vitro model of alpha-synuclein-positive glial aggregations mimics the morphology of the abnormal glial inclusions described in neuroclegenerative disorders and could be a suitable model for studying their role in the pathogenesis of these diseases. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.}},
author = {{Stefanova, N and Emgård-Mattson, Mia and Klimaschewski, L and Wenning, GK and Reindl, M}},
issn = {{0304-3940}},
keywords = {{glial cytoplasmic inclusions; ultrastructure; alpha-synuclein; glia; Lewy bodies; neurodegeneration}},
language = {{eng}},
number = {{1}},
pages = {{37--40}},
publisher = {{Elsevier}},
series = {{Neuroscience Letters}},
title = {{Ultrastructure of alpha-synuclein-positive aggregations in U373 astrocytoma and rat primary glial cells}},
url = {{http://dx.doi.org/10.1016/S0304-3940(02)00117-9}},
doi = {{10.1016/S0304-3940(02)00117-9}},
volume = {{323}},
year = {{2002}},
}