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Quantitation of immunoglobulin G by electrophoresis in agarose gel containing antibodies

Grubb, A LU orcid (1970) In Scandinavian Journal of Clinical and Laboratory Investigation 26(3). p.55-249
Abstract
It is shown that the method of protein quantitation by electrophoresis in antibody-containing agarose introduced by Laurell in 1966 can, if slightly modified, be used for quantitation of polyclonal human IgG. This modified method, permits simultaneous quantitation of IgA. It is accomplished within about 9 hours and allows comparison between the charge composition of different IgG-populations. The results agree with those obtained by single linear immunodiffusion, single radial immunodiffusion, and by electrophoresis of carbamylated sera in antibody-containing gel. It is shown that the applicability of the method is dependent upon the electrophoretic distribution of the antibodies in the antiserum used.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Antibodies, Blood Protein Electrophoresis, Gels, Humans, Immune Sera, Immunoelectrophoresis, Immunoglobulin G/analysis, Monosaccharides
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
26
issue
3
pages
55 - 249
publisher
Informa Healthcare
external identifiers
  • pmid:5477098
  • scopus:0014884395
ISSN
0036-5513
DOI
10.3109/00365517009046230
language
English
LU publication?
yes
id
1a3b7c67-ff5b-417c-af36-ed6f8316c3a3
date added to LUP
2021-10-16 18:51:54
date last changed
2024-01-12 02:56:17
@article{1a3b7c67-ff5b-417c-af36-ed6f8316c3a3,
  abstract     = {{It is shown that the method of protein quantitation by electrophoresis in antibody-containing agarose introduced by Laurell in 1966 can, if slightly modified, be used for quantitation of polyclonal human IgG. This modified method, permits simultaneous quantitation of IgA. It is accomplished within about 9 hours and allows comparison between the charge composition of different IgG-populations. The results agree with those obtained by single linear immunodiffusion, single radial immunodiffusion, and by electrophoresis of carbamylated sera in antibody-containing gel. It is shown that the applicability of the method is dependent upon the electrophoretic distribution of the antibodies in the antiserum used.}},
  author       = {{Grubb, A}},
  issn         = {{0036-5513}},
  keywords     = {{Antibodies; Blood Protein Electrophoresis; Gels; Humans; Immune Sera; Immunoelectrophoresis; Immunoglobulin G/analysis; Monosaccharides}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{55--249}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Quantitation of immunoglobulin G by electrophoresis in agarose gel containing antibodies}},
  url          = {{http://dx.doi.org/10.3109/00365517009046230}},
  doi          = {{10.3109/00365517009046230}},
  volume       = {{26}},
  year         = {{1970}},
}