A sensitive and rapid enzyme-linked immunosorbent assay using monoclonal antibodies for simultaneous quantitation of free and IgA-complexed protein HC
Fernandez-Luna, J L ; Moneo, Ignacio ; Grubb, A LU
and Méndez, E
(1985)
In Journal of Immunological Methods
82(1).
p.101-110
- Abstract
A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker... (More)
A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker could determine the concentrations of free and IgA-complexed protein HC in up to 400 serum samples in a working day.
(Less)
- author
- Fernandez-Luna, J L
; Moneo, Ignacio
; Grubb, A
LU
and Méndez, E
- organization
- publishing date
- 1985
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Alpha-Globulins/analysis, Antibodies, Monoclonal, Antibody Specificity, Antigen-Antibody Complex, Dose-Response Relationship, Immunologic, Enzyme-Linked Immunosorbent Assay/methods, Humans, Immunoglobulin A
- in
- Journal of Immunological Methods
- volume
- 82
- issue
- 1
- pages
- 101 - 110
- publisher
- Elsevier
- external identifiers
-
- scopus:0022384534
- pmid:2411818
- ISSN
- 0022-1759
- DOI
- 10.1016/0022-1759(85)90229-7
- language
- English
- LU publication?
- yes
- id
- 1aaaa339-512f-4182-a2a6-0ba091f0cc1e
- date added to LUP
- 2021-10-26 09:19:28
- date last changed
- 2024-01-12 02:55:32
@article{1aaaa339-512f-4182-a2a6-0ba091f0cc1e,
abstract = {{<p>A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker could determine the concentrations of free and IgA-complexed protein HC in up to 400 serum samples in a working day.</p>}},
author = {{Fernandez-Luna, J L and Moneo, Ignacio and Grubb, A and Méndez, E}},
issn = {{0022-1759}},
keywords = {{Alpha-Globulins/analysis; Antibodies, Monoclonal; Antibody Specificity; Antigen-Antibody Complex; Dose-Response Relationship, Immunologic; Enzyme-Linked Immunosorbent Assay/methods; Humans; Immunoglobulin A}},
language = {{eng}},
number = {{1}},
pages = {{101--110}},
publisher = {{Elsevier}},
series = {{Journal of Immunological Methods}},
title = {{A sensitive and rapid enzyme-linked immunosorbent assay using monoclonal antibodies for simultaneous quantitation of free and IgA-complexed protein HC}},
url = {{http://dx.doi.org/10.1016/0022-1759(85)90229-7}},
doi = {{10.1016/0022-1759(85)90229-7}},
volume = {{82}},
year = {{1985}},
}