Structural investigation of sulfobetaines and phospholipid monolayers at the air-water interface
Elstone, Naomi ; Arnold, Thomas ; Skoda, Maximilian W.A. ; Lewis, Simon E. ; Li, Peixun ; Hazell, Gavin and Edler, Karen J. LU
(2022)
In Physical Chemistry Chemical Physics
24(37).
p.22679-22690
- Abstract
Mixtures of sulfobetaine based lipids with phosphocholine phospholipids are of interest in order to study the interactions between zwitterionic surfactants and the phospholipids present in cell membranes. In this study we have investigated the structure of mixed monolayers of sulfobetaines and phosphocholine phospholipids. The sulfobetaine used has a single 18-carbon tail, and is referred to as SB3-18, and the phospholipid used is DMPC. Surface pressure-area isotherms of the samples were used to determine whether any phase transitions were present during the compression of the monolayers. Neutron and X-ray reflectometry were then used to investigate the structure of these monolayers perpendicular to the interface. We found that the... (More)
Mixtures of sulfobetaine based lipids with phosphocholine phospholipids are of interest in order to study the interactions between zwitterionic surfactants and the phospholipids present in cell membranes. In this study we have investigated the structure of mixed monolayers of sulfobetaines and phosphocholine phospholipids. The sulfobetaine used has a single 18-carbon tail, and is referred to as SB3-18, and the phospholipid used is DMPC. Surface pressure-area isotherms of the samples were used to determine whether any phase transitions were present during the compression of the monolayers. Neutron and X-ray reflectometry were then used to investigate the structure of these monolayers perpendicular to the interface. We found that the average headgroup and tail layer thickness was reasonably consistent across all mixtures, with a variation of less than 3 Å reported in the total thickness of the monolayers at each surface pressure. However, by selective deuteration of the two components of the monolayers, it was found that the two components have different tail layer thicknesses. For the mixture with equal compositions of DMPC and SB3-18 or with a higher composition of DMPC the tail tilts were found to be constant, resulting in a greater tail layer thickness for SB3-18 due to its longer tail. For the mixture higher in SB3-18 this was not the case, the tail tilt angle for the two components was found to be different and DMPC was found to have a greater tail layer thickness than SB3-18 as a result.
(Less)
- author
- Elstone, Naomi
; Arnold, Thomas
; Skoda, Maximilian W.A.
; Lewis, Simon E.
; Li, Peixun
; Hazell, Gavin
and Edler, Karen J.
LU
- publishing date
- 2022-09-06
- type
- Contribution to journal
- publication status
- published
- in
- Physical Chemistry Chemical Physics
- volume
- 24
- issue
- 37
- pages
- 12 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:36106535
- scopus:85138486707
- ISSN
- 1463-9076
- DOI
- 10.1039/d2cp02695c
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 The Royal Society of Chemistry.
- id
- 1aacc39f-19c4-4168-a86f-dbc4daa1843d
- date added to LUP
- 2023-01-18 08:57:44
- date last changed
- 2024-04-18 10:02:23
@article{1aacc39f-19c4-4168-a86f-dbc4daa1843d,
abstract = {{<p>Mixtures of sulfobetaine based lipids with phosphocholine phospholipids are of interest in order to study the interactions between zwitterionic surfactants and the phospholipids present in cell membranes. In this study we have investigated the structure of mixed monolayers of sulfobetaines and phosphocholine phospholipids. The sulfobetaine used has a single 18-carbon tail, and is referred to as SB3-18, and the phospholipid used is DMPC. Surface pressure-area isotherms of the samples were used to determine whether any phase transitions were present during the compression of the monolayers. Neutron and X-ray reflectometry were then used to investigate the structure of these monolayers perpendicular to the interface. We found that the average headgroup and tail layer thickness was reasonably consistent across all mixtures, with a variation of less than 3 Å reported in the total thickness of the monolayers at each surface pressure. However, by selective deuteration of the two components of the monolayers, it was found that the two components have different tail layer thicknesses. For the mixture with equal compositions of DMPC and SB3-18 or with a higher composition of DMPC the tail tilts were found to be constant, resulting in a greater tail layer thickness for SB3-18 due to its longer tail. For the mixture higher in SB3-18 this was not the case, the tail tilt angle for the two components was found to be different and DMPC was found to have a greater tail layer thickness than SB3-18 as a result.</p>}},
author = {{Elstone, Naomi and Arnold, Thomas and Skoda, Maximilian W.A. and Lewis, Simon E. and Li, Peixun and Hazell, Gavin and Edler, Karen J.}},
issn = {{1463-9076}},
language = {{eng}},
month = {{09}},
number = {{37}},
pages = {{22679--22690}},
publisher = {{Royal Society of Chemistry}},
series = {{Physical Chemistry Chemical Physics}},
title = {{Structural investigation of sulfobetaines and phospholipid monolayers at the air-water interface}},
url = {{http://dx.doi.org/10.1039/d2cp02695c}},
doi = {{10.1039/d2cp02695c}},
volume = {{24}},
year = {{2022}},
}