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Single cell analysis of proliferation and movement of cancer and normal-like cells on nanowire array substrates

Li, Zhen LU ; Kamlund, Sofia LU ; Ryser, Till; Lard, Mercy LU ; Oredsson, Stina LU and Prinz, Christelle N. LU (2018) In Journal of Materials Chemistry B 6(43). p.7042-7049
Abstract

Nanowires are presently investigated in the context of various biological and medical applications. In general, these studies are population-based, which results in sub-populations being overlooked. Here, we present a single cell analysis of cell cycle and cell movement parameters of cells seeded on nanowires using digital holographic microscopy for time-lapse imaging. MCF10A normal-like human breast epithelial cells and JIMT-1 breast cancer cells were seeded on glass, flat gallium phosphide (GaP), and on vertical GaP nanowire arrays. The cells were monitored individually using digital holographic microscopy for 48 h. The data show that cell division is affected in cells seeded on flat GaP and nanowires compared to glass, with much... (More)

Nanowires are presently investigated in the context of various biological and medical applications. In general, these studies are population-based, which results in sub-populations being overlooked. Here, we present a single cell analysis of cell cycle and cell movement parameters of cells seeded on nanowires using digital holographic microscopy for time-lapse imaging. MCF10A normal-like human breast epithelial cells and JIMT-1 breast cancer cells were seeded on glass, flat gallium phosphide (GaP), and on vertical GaP nanowire arrays. The cells were monitored individually using digital holographic microscopy for 48 h. The data show that cell division is affected in cells seeded on flat GaP and nanowires compared to glass, with much fewer cells dividing on the former two substrates compared to the latter. However, MCF10 cells that are dividing on glass and flat GaP substrates have similar cell cycle time, suggesting that distinct cell subpopulations are affected differently by the substrates. Altogether, the data highlight the importance of performing single cell analysis to increase our understanding of the versatility of cell behavior on different substrates, which is relevant in the design of nanowire applications.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Materials Chemistry B
volume
6
issue
43
pages
8 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85056353497
ISSN
2050-7518
DOI
10.1039/c8tb02049c
language
English
LU publication?
yes
id
1aecb56b-b2e9-4c9d-8f35-1d8350e39616
date added to LUP
2018-11-23 08:03:16
date last changed
2019-01-06 14:16:56
@article{1aecb56b-b2e9-4c9d-8f35-1d8350e39616,
  abstract     = {<p>Nanowires are presently investigated in the context of various biological and medical applications. In general, these studies are population-based, which results in sub-populations being overlooked. Here, we present a single cell analysis of cell cycle and cell movement parameters of cells seeded on nanowires using digital holographic microscopy for time-lapse imaging. MCF10A normal-like human breast epithelial cells and JIMT-1 breast cancer cells were seeded on glass, flat gallium phosphide (GaP), and on vertical GaP nanowire arrays. The cells were monitored individually using digital holographic microscopy for 48 h. The data show that cell division is affected in cells seeded on flat GaP and nanowires compared to glass, with much fewer cells dividing on the former two substrates compared to the latter. However, MCF10 cells that are dividing on glass and flat GaP substrates have similar cell cycle time, suggesting that distinct cell subpopulations are affected differently by the substrates. Altogether, the data highlight the importance of performing single cell analysis to increase our understanding of the versatility of cell behavior on different substrates, which is relevant in the design of nanowire applications.</p>},
  author       = {Li, Zhen and Kamlund, Sofia and Ryser, Till and Lard, Mercy and Oredsson, Stina and Prinz, Christelle N.},
  issn         = {2050-7518},
  language     = {eng},
  number       = {43},
  pages        = {7042--7049},
  publisher    = {Royal Society of Chemistry},
  series       = {Journal of Materials Chemistry B},
  title        = {Single cell analysis of proliferation and movement of cancer and normal-like cells on nanowire array substrates},
  url          = {http://dx.doi.org/10.1039/c8tb02049c},
  volume       = {6},
  year         = {2018},
}