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Impaired contraction and decreased detrusor innervation in a female rat model of pelvic neuropraxia

Hannan, Johanna L.; Powers, Shelby A.; Wang, Vinson M.; Castiglione, Fabio; Hedlund, Petter LU and Bivalacqua, Trinity J. (2017) In International Urogynecology Journal 28(7). p.1049-1056
Abstract

Introduction and hypothesis: Bilateral pelvic nerve injury (BPNI) is a model of post-radical hysterectomy neuropraxia, a common sequela. This study assessed the time course of changes to detrusor autonomic innervation, smooth muscle (SM) content and cholinergic-mediated contraction post-BPNI. Methods: Female Sprague–Dawley rats underwent BPNI or sham surgery and were evaluated 3, 7, 14, and 30 days post-BPNI (n = 8/group). Electrical field-stimulated (EFS) and carbachol-induced contractions were measured. Gene expression was assessed by qPCR for muscarinic receptor types 2 (M2) and 3 (M3), collagen type 1α1 and 3α1, and SM actin. Western blots measured M2 and M3 protein expression. Bladder sections were stained with Masson’s trichrome... (More)

Introduction and hypothesis: Bilateral pelvic nerve injury (BPNI) is a model of post-radical hysterectomy neuropraxia, a common sequela. This study assessed the time course of changes to detrusor autonomic innervation, smooth muscle (SM) content and cholinergic-mediated contraction post-BPNI. Methods: Female Sprague–Dawley rats underwent BPNI or sham surgery and were evaluated 3, 7, 14, and 30 days post-BPNI (n = 8/group). Electrical field-stimulated (EFS) and carbachol-induced contractions were measured. Gene expression was assessed by qPCR for muscarinic receptor types 2 (M2) and 3 (M3), collagen type 1α1 and 3α1, and SM actin. Western blots measured M2 and M3 protein expression. Bladder sections were stained with Masson’s trichrome for SM content and immunofluorescence staining for nerve terminals expressing vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), and neuronal nitric oxide synthase (nNOS). Results: Bilateral pelvic nerve injury caused larger bladders with less SM content and increased collagen type 1α1 and 3α1 gene expression. At early time points, cholinergic-mediated contraction increased, whereas EFS-mediated contraction decreased and returned to baseline by 30 days. Protein and gene expression of M3 was decreased 3 and 7 days post-BPNI, whereas M2 was unchanged. TH nerve terminals surrounding the detrusor decreased in all BPNI groups, whereas VAChT and nNOS terminals decreased 14 and 30 days post-BPNI. Conclusions: Bilateral pelvic nerve injury increased bladder size, impaired contractility, and decreased SM and autonomic innervation. Therapeutic strategies preventing nerve injury-mediated decline in neuronal input and SM content may prevent the development of a neurogenic bladder and improve quality of life after invasive pelvic surgery.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Autonomic innervation, Detrusor, Neurogenic bladder, Neuropraxia, Radical hysterectomy
in
International Urogynecology Journal
volume
28
issue
7
pages
1049 - 1056
publisher
Springer
external identifiers
  • scopus:85006364705
  • wos:000404157400013
ISSN
0937-3462
DOI
10.1007/s00192-016-3223-1
language
English
LU publication?
yes
id
1af5b1a8-10df-4936-a9c7-afda132e1f9a
date added to LUP
2017-01-11 13:27:38
date last changed
2018-01-07 11:44:13
@article{1af5b1a8-10df-4936-a9c7-afda132e1f9a,
  abstract     = {<p>Introduction and hypothesis: Bilateral pelvic nerve injury (BPNI) is a model of post-radical hysterectomy neuropraxia, a common sequela. This study assessed the time course of changes to detrusor autonomic innervation, smooth muscle (SM) content and cholinergic-mediated contraction post-BPNI. Methods: Female Sprague–Dawley rats underwent BPNI or sham surgery and were evaluated 3, 7, 14, and 30 days post-BPNI (n = 8/group). Electrical field-stimulated (EFS) and carbachol-induced contractions were measured. Gene expression was assessed by qPCR for muscarinic receptor types 2 (M2) and 3 (M3), collagen type 1α1 and 3α1, and SM actin. Western blots measured M2 and M3 protein expression. Bladder sections were stained with Masson’s trichrome for SM content and immunofluorescence staining for nerve terminals expressing vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), and neuronal nitric oxide synthase (nNOS). Results: Bilateral pelvic nerve injury caused larger bladders with less SM content and increased collagen type 1α1 and 3α1 gene expression. At early time points, cholinergic-mediated contraction increased, whereas EFS-mediated contraction decreased and returned to baseline by 30 days. Protein and gene expression of M3 was decreased 3 and 7 days post-BPNI, whereas M2 was unchanged. TH nerve terminals surrounding the detrusor decreased in all BPNI groups, whereas VAChT and nNOS terminals decreased 14 and 30 days post-BPNI. Conclusions: Bilateral pelvic nerve injury increased bladder size, impaired contractility, and decreased SM and autonomic innervation. Therapeutic strategies preventing nerve injury-mediated decline in neuronal input and SM content may prevent the development of a neurogenic bladder and improve quality of life after invasive pelvic surgery.</p>},
  author       = {Hannan, Johanna L. and Powers, Shelby A. and Wang, Vinson M. and Castiglione, Fabio and Hedlund, Petter and Bivalacqua, Trinity J.},
  issn         = {0937-3462},
  keyword      = {Autonomic innervation,Detrusor,Neurogenic bladder,Neuropraxia,Radical hysterectomy},
  language     = {eng},
  number       = {7},
  pages        = {1049--1056},
  publisher    = {Springer},
  series       = {International Urogynecology Journal},
  title        = {Impaired contraction and decreased detrusor innervation in a female rat model of pelvic neuropraxia},
  url          = {http://dx.doi.org/10.1007/s00192-016-3223-1},
  volume       = {28},
  year         = {2017},
}