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Hybrid Theranostic Cubosomes for Efficient NIR-Induced Photodynamic Therapy

Bazylińska, Urszula ; Wawrzyńczyk, Dominika ; Kulbacka, Julita ; Picci, Giacomo ; Manni, Livia Salvati ; Handschin, Stephan ; Fornasier, Marco LU orcid ; Caltagirone, Claudia ; Mezzenga, Raffaele and Murgia, Sergio (2022) In ACS Nano 16(4). p.5427-5438
Abstract

In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and... (More)

In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and NIR-induced photodynamic therapy were evaluated in vitro against human skin melanoma (MeWo) and ovarian (SKOV-3) cancer cells. Results evidenced the preferential uptake of the theranostic cubosomes in SKOV-3 cells in comparison to uptake in MeWo cells, and this effect was enhanced by the folic acid functionalization of the cubosomes surface. Nanocarriers coloaded with the hybrid fluorophores exhibited a superior NIR-induced photodynamic activity, also confirmed by the improved mitochondrial activity and the most affecting f-actin fibers of cytoskeleton. Similar results, but with higher photocytotoxicity, were detected when folic acid-functionalized cubosomes were incubated with SKOV-3 cells. Taken on the whole, these results prove these hybrid cubosomes are good candidates for the photodynamic treatment of tumor lesions.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
antitumor activity, daunorubicin, layer-by-layer, lipid bicontinuous cubic liquid-crystalline nanoparticles, MeWo cells, SKOV-3 cells, up-converting nanocrystals
in
ACS Nano
volume
16
issue
4
pages
12 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85127618677
  • pmid:35333516
ISSN
1936-0851
DOI
10.1021/acsnano.1c09367
language
English
LU publication?
yes
id
1b0d13fb-971d-485d-93e3-8dd94d44ccd4
date added to LUP
2022-06-14 12:15:18
date last changed
2024-06-28 10:27:15
@article{1b0d13fb-971d-485d-93e3-8dd94d44ccd4,
  abstract     = {{<p>In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and NIR-induced photodynamic therapy were evaluated in vitro against human skin melanoma (MeWo) and ovarian (SKOV-3) cancer cells. Results evidenced the preferential uptake of the theranostic cubosomes in SKOV-3 cells in comparison to uptake in MeWo cells, and this effect was enhanced by the folic acid functionalization of the cubosomes surface. Nanocarriers coloaded with the hybrid fluorophores exhibited a superior NIR-induced photodynamic activity, also confirmed by the improved mitochondrial activity and the most affecting f-actin fibers of cytoskeleton. Similar results, but with higher photocytotoxicity, were detected when folic acid-functionalized cubosomes were incubated with SKOV-3 cells. Taken on the whole, these results prove these hybrid cubosomes are good candidates for the photodynamic treatment of tumor lesions. </p>}},
  author       = {{Bazylińska, Urszula and Wawrzyńczyk, Dominika and Kulbacka, Julita and Picci, Giacomo and Manni, Livia Salvati and Handschin, Stephan and Fornasier, Marco and Caltagirone, Claudia and Mezzenga, Raffaele and Murgia, Sergio}},
  issn         = {{1936-0851}},
  keywords     = {{antitumor activity; daunorubicin; layer-by-layer; lipid bicontinuous cubic liquid-crystalline nanoparticles; MeWo cells; SKOV-3 cells; up-converting nanocrystals}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{5427--5438}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Nano}},
  title        = {{Hybrid Theranostic Cubosomes for Efficient NIR-Induced Photodynamic Therapy}},
  url          = {{http://dx.doi.org/10.1021/acsnano.1c09367}},
  doi          = {{10.1021/acsnano.1c09367}},
  volume       = {{16}},
  year         = {{2022}},
}