Hybrid Theranostic Cubosomes for Efficient NIR-Induced Photodynamic Therapy
Bazylińska, Urszula ; Wawrzyńczyk, Dominika ; Kulbacka, Julita ; Picci, Giacomo ; Manni, Livia Salvati ; Handschin, Stephan ; Fornasier, Marco LU
; Caltagirone, Claudia
; Mezzenga, Raffaele
and Murgia, Sergio
(2022)
In ACS Nano
16(4).
p.5427-5438
- Abstract
In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and... (More)
In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and NIR-induced photodynamic therapy were evaluated in vitro against human skin melanoma (MeWo) and ovarian (SKOV-3) cancer cells. Results evidenced the preferential uptake of the theranostic cubosomes in SKOV-3 cells in comparison to uptake in MeWo cells, and this effect was enhanced by the folic acid functionalization of the cubosomes surface. Nanocarriers coloaded with the hybrid fluorophores exhibited a superior NIR-induced photodynamic activity, also confirmed by the improved mitochondrial activity and the most affecting f-actin fibers of cytoskeleton. Similar results, but with higher photocytotoxicity, were detected when folic acid-functionalized cubosomes were incubated with SKOV-3 cells. Taken on the whole, these results prove these hybrid cubosomes are good candidates for the photodynamic treatment of tumor lesions.
(Less)
- author
- Bazylińska, Urszula
; Wawrzyńczyk, Dominika
; Kulbacka, Julita
; Picci, Giacomo
; Manni, Livia Salvati
; Handschin, Stephan
; Fornasier, Marco
LU
; Caltagirone, Claudia
; Mezzenga, Raffaele
and Murgia, Sergio
- organization
- publishing date
- 2022-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- antitumor activity, daunorubicin, layer-by-layer, lipid bicontinuous cubic liquid-crystalline nanoparticles, MeWo cells, SKOV-3 cells, up-converting nanocrystals
- in
- ACS Nano
- volume
- 16
- issue
- 4
- pages
- 12 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85127618677
- pmid:35333516
- ISSN
- 1936-0851
- DOI
- 10.1021/acsnano.1c09367
- language
- English
- LU publication?
- yes
- id
- 1b0d13fb-971d-485d-93e3-8dd94d44ccd4
- date added to LUP
- 2022-06-14 12:15:18
- date last changed
- 2024-07-11 07:58:56
@article{1b0d13fb-971d-485d-93e3-8dd94d44ccd4,
abstract = {{<p>In recent years, lipid bicontinuous cubic liquid-crystalline nanoparticles known as cubosomes have been under investigation because of their favorable properties as drug nanocarriers useful for anticancer treatments. Herein, we present organic/inorganic hybrid, theranostic cubosomes stabilized in water with a shell of alternate layers of chitosan, single strand DNA (model genetic material for potential gene therapy), and folic acid-chitosan conjugate (the outmost layer), coencapsulating up-converting Er3+ and Yb3+ codoped NaYF4 nanoparticles and daunorubicin. The latter acts as a chemotherapeutic drug of photosensitizing activity, while up-converting nanoparticles serve as energy harvester and diagnostic agent. Cellular uptake and NIR-induced photodynamic therapy were evaluated in vitro against human skin melanoma (MeWo) and ovarian (SKOV-3) cancer cells. Results evidenced the preferential uptake of the theranostic cubosomes in SKOV-3 cells in comparison to uptake in MeWo cells, and this effect was enhanced by the folic acid functionalization of the cubosomes surface. Nanocarriers coloaded with the hybrid fluorophores exhibited a superior NIR-induced photodynamic activity, also confirmed by the improved mitochondrial activity and the most affecting f-actin fibers of cytoskeleton. Similar results, but with higher photocytotoxicity, were detected when folic acid-functionalized cubosomes were incubated with SKOV-3 cells. Taken on the whole, these results prove these hybrid cubosomes are good candidates for the photodynamic treatment of tumor lesions. </p>}},
author = {{Bazylińska, Urszula and Wawrzyńczyk, Dominika and Kulbacka, Julita and Picci, Giacomo and Manni, Livia Salvati and Handschin, Stephan and Fornasier, Marco and Caltagirone, Claudia and Mezzenga, Raffaele and Murgia, Sergio}},
issn = {{1936-0851}},
keywords = {{antitumor activity; daunorubicin; layer-by-layer; lipid bicontinuous cubic liquid-crystalline nanoparticles; MeWo cells; SKOV-3 cells; up-converting nanocrystals}},
language = {{eng}},
number = {{4}},
pages = {{5427--5438}},
publisher = {{The American Chemical Society (ACS)}},
series = {{ACS Nano}},
title = {{Hybrid Theranostic Cubosomes for Efficient NIR-Induced Photodynamic Therapy}},
url = {{http://dx.doi.org/10.1021/acsnano.1c09367}},
doi = {{10.1021/acsnano.1c09367}},
volume = {{16}},
year = {{2022}},
}