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Accelerated wound closure - Differently organized nanofibers affect cell migration and hence the closure of artificial wounds in a cell based in vitro model

Ottosson, Maximilian LU ; Jakobsson, Albin LU and Johansson, Fredrik LU (2017) In PLoS ONE 12(1).
Abstract

Nanofiber meshes holds great promise in wound healing applications by mimicking the topography of extracellular matrix, hence providing guidance for crucial cells involved in the regenerative processes. Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-å-caprolactone scaffolds with different nanofiber orientation. Fibroblasts were cultured to confluency for 24h before custom-made inserts were removed, creating cell-free zones serving as artificial wounds. Cell migration into these wounds was evaluated at 0-, 48- and 96h. Cell morphological analysis was performed using nuclei- and cytoskeleton stainings. Cell viability was assessed using a... (More)

Nanofiber meshes holds great promise in wound healing applications by mimicking the topography of extracellular matrix, hence providing guidance for crucial cells involved in the regenerative processes. Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-å-caprolactone scaffolds with different nanofiber orientation. Fibroblasts were cultured to confluency for 24h before custom-made inserts were removed, creating cell-free zones serving as artificial wounds. Cell migration into these wounds was evaluated at 0-, 48- and 96h. Cell morphological analysis was performed using nuclei- and cytoskeleton stainings. Cell viability was assessed using a biochemical assay. This study demonstrates a novel in vitro wound assay, for exploring of the impact of nanofibers on wound healing. Additionally we show that it's possible to affect the process of wound closure in a spatial manner using nanotopographies, resulting in faster closure on aligned fiber substrates.

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type
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publication status
published
subject
in
PLoS ONE
volume
12
issue
1
publisher
Public Library of Science
external identifiers
  • scopus:85009126859
  • wos:000391641500089
ISSN
1932-6203
DOI
10.1371/journal.pone.0169419
language
English
LU publication?
yes
id
1b9fd36b-44db-4d69-a3ab-6600dcae4ebf
date added to LUP
2017-02-06 11:56:59
date last changed
2018-01-07 11:48:44
@article{1b9fd36b-44db-4d69-a3ab-6600dcae4ebf,
  abstract     = {<p>Nanofiber meshes holds great promise in wound healing applications by mimicking the topography of extracellular matrix, hence providing guidance for crucial cells involved in the regenerative processes. Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-å-caprolactone scaffolds with different nanofiber orientation. Fibroblasts were cultured to confluency for 24h before custom-made inserts were removed, creating cell-free zones serving as artificial wounds. Cell migration into these wounds was evaluated at 0-, 48- and 96h. Cell morphological analysis was performed using nuclei- and cytoskeleton stainings. Cell viability was assessed using a biochemical assay. This study demonstrates a novel in vitro wound assay, for exploring of the impact of nanofibers on wound healing. Additionally we show that it's possible to affect the process of wound closure in a spatial manner using nanotopographies, resulting in faster closure on aligned fiber substrates.</p>},
  articleno    = {e0169419},
  author       = {Ottosson, Maximilian and Jakobsson, Albin and Johansson, Fredrik},
  issn         = {1932-6203},
  language     = {eng},
  month        = {01},
  number       = {1},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Accelerated wound closure - Differently organized nanofibers affect cell migration and hence the closure of artificial wounds in a cell based in vitro model},
  url          = {http://dx.doi.org/10.1371/journal.pone.0169419},
  volume       = {12},
  year         = {2017},
}