Study of in Vitro and in Vivo Bone Formation in Composite Cryogels and the Influence of Electrical Stimulation.
(2015) In International Journal of Biological Sciences 11(11). p.1325-1336- Abstract
- This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further,... (More)
- This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further, osteogenic differentiation of murine mesenchymal stem cells was studied with and without electrical stimulation. The q-PCR analysis shows that the electrically stimulated cryogels exhibit ~ 6 folds higher collagen type I and ~ 10 folds higher osteopontin mRNA level, in comparison to the unstimulated cryogels. Thus, PTAC biocomposite cryogels present osteoinductive and osteoconductive properties during in vitro and in vivo studies and support osteogenic differentiation of mesenchymal stem cells under the influence of electrical stimulation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8243177
- author
- Mishra, Ruchi ; Raina, Deepak LU ; Pelkonen, Mea LU ; Lidgren, Lars LU ; Tägil, Magnus LU and Kumar, Ashok
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Biological Sciences
- volume
- 11
- issue
- 11
- pages
- 1325 - 1336
- publisher
- Ivyspring International Publisher
- external identifiers
-
- pmid:26535027
- wos:000363677500009
- scopus:84945309162
- pmid:26535027
- ISSN
- 1449-2288
- DOI
- 10.7150/ijbs.13139
- language
- English
- LU publication?
- yes
- id
- 1ba1b8d7-c8f1-4421-b209-f9b1c3116782 (old id 8243177)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26535027?dopt=Abstract
- date added to LUP
- 2016-04-01 15:05:08
- date last changed
- 2022-04-22 06:47:41
@article{1ba1b8d7-c8f1-4421-b209-f9b1c3116782, abstract = {{This work studies osteoinduction and bone conduction in polyvinyl alcohol-tetraethylorthosilicate-alginate-calcium oxide (PTAC) biocomposite cryogels along with the synergistic effect of electrical stimulation. In vitro osteoinduction of C2C12 myoblast towards osteogenic lineage is demonstrated through alkaline phosphatase assay, scanning electron microscopy and energy dispersive X-ray spectroscopy. These results were followed by in vivo implantation studies of PTAC biocomposite cryogel scaffolds in the bone conduction chamber model depicting bone formation after 24 days based on immunohistological staining for osteogenic markers, i.e., collagen type I (Col I), osteocalcin (OCN), osteopontin (OPN) and bone sialoprotein (BSP). Further, osteogenic differentiation of murine mesenchymal stem cells was studied with and without electrical stimulation. The q-PCR analysis shows that the electrically stimulated cryogels exhibit ~ 6 folds higher collagen type I and ~ 10 folds higher osteopontin mRNA level, in comparison to the unstimulated cryogels. Thus, PTAC biocomposite cryogels present osteoinductive and osteoconductive properties during in vitro and in vivo studies and support osteogenic differentiation of mesenchymal stem cells under the influence of electrical stimulation.}}, author = {{Mishra, Ruchi and Raina, Deepak and Pelkonen, Mea and Lidgren, Lars and Tägil, Magnus and Kumar, Ashok}}, issn = {{1449-2288}}, language = {{eng}}, number = {{11}}, pages = {{1325--1336}}, publisher = {{Ivyspring International Publisher}}, series = {{International Journal of Biological Sciences}}, title = {{Study of in Vitro and in Vivo Bone Formation in Composite Cryogels and the Influence of Electrical Stimulation.}}, url = {{http://dx.doi.org/10.7150/ijbs.13139}}, doi = {{10.7150/ijbs.13139}}, volume = {{11}}, year = {{2015}}, }