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Thermoresponsive Polymer Brushes on Organic Microspheres for Biomolecular Separation and Immobilization

Bagan Navarro, Hector LU ; Kamra, Tripta; Jiang, Lingdong LU and Ye, Lei LU (2017) In Macromolecular Chemistry and Physics 218(3).
Abstract

A new method is developed to synthesize organic microsphere-supported polymer brushes for affinity separation of saccharides and glycoproteins. In the first step, crosslinked polymer microspheres are synthesized using atom transfer radical polymerization (ATRP). The terminal ATRP initiators on the microspheres are used to graft polymer brushes from propargyl acrylate and N-isopropyl acrylamide. The microsphere-supported polymer brushes are conjugated with an azide-tagged phenylboronic acid to complete the material synthesis. The microsphere-supported, boronic acid-containing polymer brushes are able to bind not only low molecular weight cis-diol compounds but also glycoproteins. The synthetic procedure developed in this work provides a... (More)

A new method is developed to synthesize organic microsphere-supported polymer brushes for affinity separation of saccharides and glycoproteins. In the first step, crosslinked polymer microspheres are synthesized using atom transfer radical polymerization (ATRP). The terminal ATRP initiators on the microspheres are used to graft polymer brushes from propargyl acrylate and N-isopropyl acrylamide. The microsphere-supported polymer brushes are conjugated with an azide-tagged phenylboronic acid to complete the material synthesis. The microsphere-supported, boronic acid-containing polymer brushes are able to bind not only low molecular weight cis-diol compounds but also glycoproteins. The synthetic procedure developed in this work provides a convenient means for preparing all-organic microsphere-supported polymer brushes that have high alkaline stability. Using the surface-attached polymer brushes to immobilize a catalytic glycoprotein (horseradish peroxidase), it is possible to retain on-particle enzyme activity due to the open structure of the polymer brushes and the oriented immobilization. (Figure presented.).

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organization
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type
Contribution to journal
publication status
published
subject
keywords
atom transfer radical polymerization, bioseparation, boronic acid, glycoprotein, polymer brush
in
Macromolecular Chemistry and Physics
volume
218
issue
3
publisher
John Wiley & Sons
external identifiers
  • scopus:85002727012
  • wos:000394704000007
ISSN
1022-1352
DOI
10.1002/macp.201600432
language
English
LU publication?
yes
id
1bd8bca9-4cb1-47be-b883-6aefb1320cd6
date added to LUP
2017-02-20 10:17:04
date last changed
2018-01-07 11:51:05
@article{1bd8bca9-4cb1-47be-b883-6aefb1320cd6,
  abstract     = {<p>A new method is developed to synthesize organic microsphere-supported polymer brushes for affinity separation of saccharides and glycoproteins. In the first step, crosslinked polymer microspheres are synthesized using atom transfer radical polymerization (ATRP). The terminal ATRP initiators on the microspheres are used to graft polymer brushes from propargyl acrylate and N-isopropyl acrylamide. The microsphere-supported polymer brushes are conjugated with an azide-tagged phenylboronic acid to complete the material synthesis. The microsphere-supported, boronic acid-containing polymer brushes are able to bind not only low molecular weight cis-diol compounds but also glycoproteins. The synthetic procedure developed in this work provides a convenient means for preparing all-organic microsphere-supported polymer brushes that have high alkaline stability. Using the surface-attached polymer brushes to immobilize a catalytic glycoprotein (horseradish peroxidase), it is possible to retain on-particle enzyme activity due to the open structure of the polymer brushes and the oriented immobilization. (Figure presented.).</p>},
  articleno    = {1600432},
  author       = {Bagan Navarro, Hector and Kamra, Tripta and Jiang, Lingdong and Ye, Lei},
  issn         = {1022-1352},
  keyword      = {atom transfer radical polymerization,bioseparation,boronic acid,glycoprotein,polymer brush},
  language     = {eng},
  month        = {02},
  number       = {3},
  publisher    = {John Wiley & Sons},
  series       = {Macromolecular Chemistry and Physics},
  title        = {Thermoresponsive Polymer Brushes on Organic Microspheres for Biomolecular Separation and Immobilization},
  url          = {http://dx.doi.org/10.1002/macp.201600432},
  volume       = {218},
  year         = {2017},
}