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Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy

Schweiger, P F ; Rouhier, Hervé LU and Söderström, Bengt LU (2002) In Mycological Research 106(3). p.349-354
Abstract
A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research
volume
106
issue
3
pages
349 - 354
publisher
Cambridge University Press
external identifiers
  • wos:000176325400013
  • scopus:0036276542
ISSN
0953-7562
DOI
10.1017/S0953756202005579
language
English
LU publication?
yes
id
1c1dc5d3-e578-4c26-8c94-ae1420466449 (old id 146907)
date added to LUP
2016-04-01 12:16:18
date last changed
2024-02-07 03:42:00
@article{1c1dc5d3-e578-4c26-8c94-ae1420466449,
  abstract     = {{A method for the observation of the three-dimensional structure of intact ectomycorrhizal rhizomorphs is described. The method is based on a combination of clearing the material with KOH followed by staining with congo red and subsequent imaging under a laser scanning confocal microscope (LSCM). The images obtained are of a much higher three-dimensional resolution than those obtained previously by use of conventional light microscopical techniques. The structure of highly differentiated and undifferentiated rhizomorphs is described. Applications of the method are briefly discussed.}},
  author       = {{Schweiger, P F and Rouhier, Hervé and Söderström, Bengt}},
  issn         = {{0953-7562}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{349--354}},
  publisher    = {{Cambridge University Press}},
  series       = {{Mycological Research}},
  title        = {{Visualisation of ectomycorrhizal rhizomorph structure using laser scanning confocal microscopy}},
  url          = {{https://lup.lub.lu.se/search/files/2854472/625109.pdf}},
  doi          = {{10.1017/S0953756202005579}},
  volume       = {{106}},
  year         = {{2002}},
}