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Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue

Zhao, Yuan ; Uduman, Mohamed ; Siu, Jacqueline H.Y. ; Tull, Thomas J. ; Sanderson, Jeremy D. ; Wu, Yu Chang Bryan ; Zhou, Julian Q. ; Petrov, Nedyalko ; Ellis, Richard and Todd, Katrina , et al. (2018) In Nature Communications 9(1).
Abstract

Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27−CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organised gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely... (More)

Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27−CD45RBMEM55+ population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organised gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adaptive immunity, B cells, Immunological memory, Lymphocyte differentiation
in
Nature Communications
volume
9
issue
1
article number
3857
pages
15 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:30242242
  • scopus:85053755692
ISSN
2041-1723
DOI
10.1038/s41467-018-06089-1
language
English
LU publication?
no
additional info
Funding Information: This work was funded by the Medical Research Council of Great Britain (MR/L009382/1, MR/P021964/1 and MR/R000964/1), the Gates Cambridge Trust, The St. Thomas’ Lupus Trust and the National Institute for Health Research (NIHR) Biomedical Research Centre based at Guy’s and St. Thomas’ NHS Foundation Trust and King’s College London. This work was supported by the National Institutes of Health (NIH) [R01AI104739]. The views expressed are those of the authors and not necessarily those of the NIH, NHS, the NIHR or the Department of Health. Publisher Copyright: © 2018, The Author(s).
id
1c6d6bbd-3ac2-45b2-ba7c-8b0895cdbf2e
date added to LUP
2023-11-28 10:22:54
date last changed
2024-04-25 13:09:07
@article{1c6d6bbd-3ac2-45b2-ba7c-8b0895cdbf2e,
  abstract     = {{<p>Human memory B cells and marginal zone (MZ) B cells share common features such as the expression of CD27 and somatic mutations in their IGHV and BCL6 genes, but the relationship between them is controversial. Here, we show phenotypic progression within lymphoid tissues as MZ B cells emerge from the mature naïve B cell pool via a precursor CD27<sup>−</sup>CD45RB<sup>MEM55+</sup> population distant from memory cells. By imaging mass cytometry, we find that MZ B cells and memory B cells occupy different microanatomical niches in organised gut lymphoid tissues. Both populations disseminate widely between distant lymphoid tissues and blood, and both diversify their IGHV repertoire in gut germinal centres (GC), but nevertheless remain largely clonally separate. MZ B cells are therefore not developmentally contiguous with or analogous to classical memory B cells despite their shared ability to transit through GC, where somatic mutations are acquired.</p>}},
  author       = {{Zhao, Yuan and Uduman, Mohamed and Siu, Jacqueline H.Y. and Tull, Thomas J. and Sanderson, Jeremy D. and Wu, Yu Chang Bryan and Zhou, Julian Q. and Petrov, Nedyalko and Ellis, Richard and Todd, Katrina and Chavele, Konstantia Maria and Guesdon, William and Vossenkamper, Anna and Jassem, Wayel and D’Cruz, David P. and Fear, David J. and John, Susan and Scheel-Toellner, Dagmar and Hopkins, Claire and Moreno, Estefania and Woodman, Natalie L. and Ciccarelli, Francesca and Heck, Susanne and Kleinstein, Steven H. and Bemark, Mats and Spencer, Jo}},
  issn         = {{2041-1723}},
  keywords     = {{Adaptive immunity; B cells; Immunological memory; Lymphocyte differentiation}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{Spatiotemporal segregation of human marginal zone and memory B cell populations in lymphoid tissue}},
  url          = {{http://dx.doi.org/10.1038/s41467-018-06089-1}},
  doi          = {{10.1038/s41467-018-06089-1}},
  volume       = {{9}},
  year         = {{2018}},
}