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Enhanced Direct Electron Transfer of Fructose Dehydrogenase Rationally Immobilized on a 2-Aminoanthracene Diazonium Cation Grafted Single-Walled Carbon Nanotube Based Electrode

Bollella, Paolo; Hibino, Yuya; Kano, Kenji; Gorton, Lo LU and Antiochia, Riccarda (2018) In ACS Catalysis p.10279-10289
Abstract

In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy-carbon electrode (GCE) upon which anthracene-modified single-walled carbon nanotubes were deposited. The SWCNTs were activated in situ with a diazonium salt synthesized through the reaction of 2-aminoanthracene with NaNO2 in acidic media (0.5 M HCl) for 5 min at 0 °C. After the in situ reaction, the 2-aminoanthracene diazonium salt was electrodeposited by running cyclic voltammograms from +1000 to -1000 mV. The anthracene-SWCNT-modified GCE was further incubated in an FDH solution, allowing enzyme adsorption. Cyclic voltammograms of the FDH-modified electrode revealed two couples of redox waves... (More)

In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy-carbon electrode (GCE) upon which anthracene-modified single-walled carbon nanotubes were deposited. The SWCNTs were activated in situ with a diazonium salt synthesized through the reaction of 2-aminoanthracene with NaNO2 in acidic media (0.5 M HCl) for 5 min at 0 °C. After the in situ reaction, the 2-aminoanthracene diazonium salt was electrodeposited by running cyclic voltammograms from +1000 to -1000 mV. The anthracene-SWCNT-modified GCE was further incubated in an FDH solution, allowing enzyme adsorption. Cyclic voltammograms of the FDH-modified electrode revealed two couples of redox waves possibly ascribed to the heme c1 and heme c3 of the cytochrome domain. In the presence of 10 mM fructose two catalytic waves could clearly be seen and were correlated with two heme cs (heme c1 and c2), with a maximum current density of 485 ± 21 μA cm-2 at 0.4 V at a sweep rate of 10 mV s-1. In contrast, for the plain SWCNT-modified GCE only one catalytic wave and one couple of redox waves were observed. Adsorbing FDH directly onto a GCE showed no non-turnover electrochemistry of FDH, and in the presence of fructose only a slight catalytic effect could be seen. These differences can be explained by considering the hydrophobic pocket close to heme c1, heme c2, and heme c3 of the cytochrome domain at which the anthracenyl aromatic structure could interact through π-π interactions with the aromatic side chains of the amino acids present in the hydrophobic pocket of FDH.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
2-aminoanthracene (2-ANT), diazonium coupling, fructose dehydrogenase (FDH), hydrophobic pocket, single-walled carbon nanotubes (SWCNTs)
in
ACS Catalysis
pages
11 pages
publisher
The American Chemical Society
external identifiers
  • scopus:85054852393
ISSN
2155-5435
DOI
10.1021/acscatal.8b02729
language
English
LU publication?
yes
id
1cea4860-f4d6-47e6-b832-29df306d2aad
date added to LUP
2018-11-13 10:34:00
date last changed
2019-02-20 11:35:24
@article{1cea4860-f4d6-47e6-b832-29df306d2aad,
  abstract     = {<p>In this paper, an efficient direct electron transfer (DET) reaction was achieved between fructose dehydrogenase (FDH) and a glassy-carbon electrode (GCE) upon which anthracene-modified single-walled carbon nanotubes were deposited. The SWCNTs were activated in situ with a diazonium salt synthesized through the reaction of 2-aminoanthracene with NaNO<sub>2</sub> in acidic media (0.5 M HCl) for 5 min at 0 °C. After the in situ reaction, the 2-aminoanthracene diazonium salt was electrodeposited by running cyclic voltammograms from +1000 to -1000 mV. The anthracene-SWCNT-modified GCE was further incubated in an FDH solution, allowing enzyme adsorption. Cyclic voltammograms of the FDH-modified electrode revealed two couples of redox waves possibly ascribed to the heme c<sub>1</sub> and heme c<sub>3</sub> of the cytochrome domain. In the presence of 10 mM fructose two catalytic waves could clearly be seen and were correlated with two heme cs (heme c<sub>1</sub> and c<sub>2</sub>), with a maximum current density of 485 ± 21 μA cm<sup>-2</sup> at 0.4 V at a sweep rate of 10 mV s<sup>-1</sup>. In contrast, for the plain SWCNT-modified GCE only one catalytic wave and one couple of redox waves were observed. Adsorbing FDH directly onto a GCE showed no non-turnover electrochemistry of FDH, and in the presence of fructose only a slight catalytic effect could be seen. These differences can be explained by considering the hydrophobic pocket close to heme c<sub>1</sub>, heme c<sub>2</sub>, and heme c<sub>3</sub> of the cytochrome domain at which the anthracenyl aromatic structure could interact through π-π interactions with the aromatic side chains of the amino acids present in the hydrophobic pocket of FDH.</p>},
  author       = {Bollella, Paolo and Hibino, Yuya and Kano, Kenji and Gorton, Lo and Antiochia, Riccarda},
  issn         = {2155-5435},
  keyword      = {2-aminoanthracene (2-ANT),diazonium coupling,fructose dehydrogenase (FDH),hydrophobic pocket,single-walled carbon nanotubes (SWCNTs)},
  language     = {eng},
  pages        = {10279--10289},
  publisher    = {The American Chemical Society},
  series       = {ACS Catalysis},
  title        = {Enhanced Direct Electron Transfer of Fructose Dehydrogenase Rationally Immobilized on a 2-Aminoanthracene Diazonium Cation Grafted Single-Walled Carbon Nanotube Based Electrode},
  url          = {http://dx.doi.org/10.1021/acscatal.8b02729},
  year         = {2018},
}