Intrathecal release of nitric oxide and its relation to final brain damage in patients with stroke
(2000) In Cerebrovascular Diseases 10(3). p.6-200- Abstract
The potential role of inflammatory mechanisms in the pathophysiology of ischemic brain damage has intensely been discussed. We have recently demonstrated that stroke patients display an intrathecal production of proinflammatory cytokines early after onset of symptoms. IL-1beta, one of these cytokines, stimulates the production of nitric oxide (NO), a potent inflammatory mediator. The aim of the present study was to investigate the intrathecal levels of nitrate, one of the main metabolites of NO in acute stroke and to relate its levels to brain damage. Stroke patients were prospectively studied with clinical evaluation, radiological assessment and analysis of intrathecal levels of nitrate by gas chromatography/mass spectrometry. In... (More)
The potential role of inflammatory mechanisms in the pathophysiology of ischemic brain damage has intensely been discussed. We have recently demonstrated that stroke patients display an intrathecal production of proinflammatory cytokines early after onset of symptoms. IL-1beta, one of these cytokines, stimulates the production of nitric oxide (NO), a potent inflammatory mediator. The aim of the present study was to investigate the intrathecal levels of nitrate, one of the main metabolites of NO in acute stroke and to relate its levels to brain damage. Stroke patients were prospectively studied with clinical evaluation, radiological assessment and analysis of intrathecal levels of nitrate by gas chromatography/mass spectrometry. In addition, simultaneous analyses of cytokines and of soluble Fas/APO-1 and bcl-2, two proteins regulating apoptosis, were performed. The intrathecal levels of nitrate were not significantly different in stroke patients compared to controls throughout the observation period. However, the intrathecal levels of nitrate increased significantly 3 months after stroke onset compared with the first 3 days. Interestingly, the levels of nitrate measured at stroke onset were negatively correlated to the final size of infarct volume (r = -0.69, p < 0.05) measured by MRI. In addition, patients with large infarcts displayed significantly (p = 0.008) lower levels of nitrate in cerebrospinal fluid compared to patients with small infarcts during the first 3 days after stroke onset. In contrast, the intrathecal levels of nitrate were significantly positively correlated (r = 0.79, p < 0. 001) to the neurological deficit and negatively correlated (r = -0. 76, p < 0.05) to bcl-2, a protein downregulating neuronal apoptosis, in the late stage of the stroke. Early NO production is associated with a smaller infarct volume, suggesting a protective effect, whereas late NO production is associated with severer neurological deficits, suggesting a neurotoxic effect. Treatment trials pertaining to modulate NO production in stroke should take into consideration the dual effect of NO on ischemic brain damage.
(Less)
- author
- Tarkowski, E ; Ringqvist, Å LU ; Rosengren, L ; Jensen, C ; Ekholm, S and Wennmalm, Å
- publishing date
- 2000-04-25
- type
- Contribution to journal
- publication status
- published
- keywords
- Adult, Aged, Apoptosis, Biomarkers/cerebrospinal fluid, Brain/diagnostic imaging, Brain Damage, Chronic/diagnostic imaging, Cytokines/cerebrospinal fluid, Female, Gas Chromatography-Mass Spectrometry, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Nitrates/cerebrospinal fluid, Nitric Oxide/cerebrospinal fluid, Reference Values, Stroke/cerebrospinal fluid, Time Factors, Tomography, X-Ray Computed, fas Receptor/cerebrospinal fluid
- in
- Cerebrovascular Diseases
- volume
- 10
- issue
- 3
- pages
- 6 - 200
- publisher
- Karger
- external identifiers
-
- scopus:0034005885
- pmid:10773646
- ISSN
- 1015-9770
- DOI
- 10.1159/000016057
- language
- English
- LU publication?
- no
- additional info
- Copyright 2000 S. Karger AG, Basel.
- id
- 1d2f46bc-6b30-41b1-9b52-6f1aa8184e1e
- date added to LUP
- 2022-05-09 14:45:29
- date last changed
- 2024-03-21 07:52:11
@article{1d2f46bc-6b30-41b1-9b52-6f1aa8184e1e,
abstract = {{<p>The potential role of inflammatory mechanisms in the pathophysiology of ischemic brain damage has intensely been discussed. We have recently demonstrated that stroke patients display an intrathecal production of proinflammatory cytokines early after onset of symptoms. IL-1beta, one of these cytokines, stimulates the production of nitric oxide (NO), a potent inflammatory mediator. The aim of the present study was to investigate the intrathecal levels of nitrate, one of the main metabolites of NO in acute stroke and to relate its levels to brain damage. Stroke patients were prospectively studied with clinical evaluation, radiological assessment and analysis of intrathecal levels of nitrate by gas chromatography/mass spectrometry. In addition, simultaneous analyses of cytokines and of soluble Fas/APO-1 and bcl-2, two proteins regulating apoptosis, were performed. The intrathecal levels of nitrate were not significantly different in stroke patients compared to controls throughout the observation period. However, the intrathecal levels of nitrate increased significantly 3 months after stroke onset compared with the first 3 days. Interestingly, the levels of nitrate measured at stroke onset were negatively correlated to the final size of infarct volume (r = -0.69, p < 0.05) measured by MRI. In addition, patients with large infarcts displayed significantly (p = 0.008) lower levels of nitrate in cerebrospinal fluid compared to patients with small infarcts during the first 3 days after stroke onset. In contrast, the intrathecal levels of nitrate were significantly positively correlated (r = 0.79, p < 0. 001) to the neurological deficit and negatively correlated (r = -0. 76, p < 0.05) to bcl-2, a protein downregulating neuronal apoptosis, in the late stage of the stroke. Early NO production is associated with a smaller infarct volume, suggesting a protective effect, whereas late NO production is associated with severer neurological deficits, suggesting a neurotoxic effect. Treatment trials pertaining to modulate NO production in stroke should take into consideration the dual effect of NO on ischemic brain damage.</p>}},
author = {{Tarkowski, E and Ringqvist, Å and Rosengren, L and Jensen, C and Ekholm, S and Wennmalm, Å}},
issn = {{1015-9770}},
keywords = {{Adult; Aged; Apoptosis; Biomarkers/cerebrospinal fluid; Brain/diagnostic imaging; Brain Damage, Chronic/diagnostic imaging; Cytokines/cerebrospinal fluid; Female; Gas Chromatography-Mass Spectrometry; Humans; Magnetic Resonance Imaging; Male; Middle Aged; Nitrates/cerebrospinal fluid; Nitric Oxide/cerebrospinal fluid; Reference Values; Stroke/cerebrospinal fluid; Time Factors; Tomography, X-Ray Computed; fas Receptor/cerebrospinal fluid}},
language = {{eng}},
month = {{04}},
number = {{3}},
pages = {{6--200}},
publisher = {{Karger}},
series = {{Cerebrovascular Diseases}},
title = {{Intrathecal release of nitric oxide and its relation to final brain damage in patients with stroke}},
url = {{http://dx.doi.org/10.1159/000016057}},
doi = {{10.1159/000016057}},
volume = {{10}},
year = {{2000}},
}