Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women
(2017) In Folia histochemica et cytobiologica 55(1). p.26-36- Abstract
INTRODUCTION: Much of what we know about the functioning of human T lymphocytes is based on the experiments carried out in atmospheric oxygen (O₂) concentrations, which are significantly higher than those maintained in blood. Interestingly, the gender differences in the activity of T cells and their susceptibility to apoptosis under different O₂ conditions have not yet been described. The aim of the study was to compare two main markers of lymphocyte function: proliferation capacity and ability to produce cytokines as well as their susceptibility to apoptosis under two different O₂ concentrations, between men and women.
MATERIALS AND METHODS: 25 healthy volunteers, both males (13) and females (12) were recruited to the study (mean... (More)
INTRODUCTION: Much of what we know about the functioning of human T lymphocytes is based on the experiments carried out in atmospheric oxygen (O₂) concentrations, which are significantly higher than those maintained in blood. Interestingly, the gender differences in the activity of T cells and their susceptibility to apoptosis under different O₂ conditions have not yet been described. The aim of the study was to compare two main markers of lymphocyte function: proliferation capacity and ability to produce cytokines as well as their susceptibility to apoptosis under two different O₂ concentrations, between men and women.
MATERIALS AND METHODS: 25 healthy volunteers, both males (13) and females (12) were recruited to the study (mean age 25.48 ± 5.51). By using cytometry proliferation parameters of human CD4+ CD28+ cells or CD8+CD28+ cells in response to polyclonal stimulation of the TCR/CD3 complex at atmospheric (21%) and physiological (10%) O₂ concentrations using our modified dividing cell tracking technique (DCT) were analyzed as well as the percentages of apoptotic cells. We also determined the levels of IFN-γ, IL-2, IL-10 and IL-17A using Cytometric Bead Array Flex system in cell culture supernatants.
RESULTS: CD4+CD28+ and CD8+CD28+ cells from the whole study group were characterized by shorter time required to enter the first (G1) phase of the first cell cycle at 21% compared to 10% O₂. Both T cell populations performed significantly more divisions at 21% O₂. The percentages of dividing cells were also significantly higher at atmospheric O₂. Interestingly, data analysis by gender showed that male lymphocytes had similar proliferative parameters at both O₂ concentrations while female lymphocytes proliferate more efficiently (note from the author: we cannot say that lymphocytes proliferate faster, rather more effectively, because cells perform more divisions, which gives more percentage of offspring cells) at 21% oxygen. Compared to males, the female CD4+ cells showed increased susceptibility to apoptosis at both O₂ concentrations. No differences in the levels of cytokines regardless of gender and oxygen conditions were found.
CONCLUSIONS: We showed that in vitro female T cells (both CD4+ and CD8+ cells) are more sensitive than male lymphocytes to low O2 concentration as demonstrated by the decrease in their proliferation dynamics. The effect does not depend on increased apoptosis of female T cells under low O₂ because percentage of apoptotic cells was similar at both O₂ concentrations.
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- author
- Waskowska, Agnieszka ; Lisowska, Katarzyna A ; Daca, Agnieszka ; Henc, Izabella ; Brandberg, Fredrik LU ; Mazurek, Paula ; Brzustewicz, Edyta ; Witkowski, Jacek M and Bryl, Ewa
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adult, Apoptosis/drug effects, CD4-CD8 Ratio, Cell Proliferation/drug effects, Cells, Cultured, Female, Humans, Lymphocyte Activation/drug effects, Male, Oxygen/pharmacology, Sex Factors, T-Lymphocytes/cytology
- in
- Folia histochemica et cytobiologica
- volume
- 55
- issue
- 1
- pages
- 26 - 36
- publisher
- Via Medica
- external identifiers
-
- scopus:85019224409
- pmid:28509314
- ISSN
- 0239-8508
- DOI
- 10.5603/FHC.a2017.0006
- language
- English
- LU publication?
- no
- id
- 1ddbe291-d663-49ef-91b6-d1136f953eb2
- date added to LUP
- 2025-01-30 09:54:43
- date last changed
- 2025-04-04 15:06:59
@article{1ddbe291-d663-49ef-91b6-d1136f953eb2,
abstract = {{<p>INTRODUCTION: Much of what we know about the functioning of human T lymphocytes is based on the experiments carried out in atmospheric oxygen (O₂) concentrations, which are significantly higher than those maintained in blood. Interestingly, the gender differences in the activity of T cells and their susceptibility to apoptosis under different O₂ conditions have not yet been described. The aim of the study was to compare two main markers of lymphocyte function: proliferation capacity and ability to produce cytokines as well as their susceptibility to apoptosis under two different O₂ concentrations, between men and women.</p><p>MATERIALS AND METHODS: 25 healthy volunteers, both males (13) and females (12) were recruited to the study (mean age 25.48 ± 5.51). By using cytometry proliferation parameters of human CD4+ CD28+ cells or CD8+CD28+ cells in response to polyclonal stimulation of the TCR/CD3 complex at atmospheric (21%) and physiological (10%) O₂ concentrations using our modified dividing cell tracking technique (DCT) were analyzed as well as the percentages of apoptotic cells. We also determined the levels of IFN-γ, IL-2, IL-10 and IL-17A using Cytometric Bead Array Flex system in cell culture supernatants.</p><p>RESULTS: CD4+CD28+ and CD8+CD28+ cells from the whole study group were characterized by shorter time required to enter the first (G1) phase of the first cell cycle at 21% compared to 10% O₂. Both T cell populations performed significantly more divisions at 21% O₂. The percentages of dividing cells were also significantly higher at atmospheric O₂. Interestingly, data analysis by gender showed that male lymphocytes had similar proliferative parameters at both O₂ concentrations while female lymphocytes proliferate more efficiently (note from the author: we cannot say that lymphocytes proliferate faster, rather more effectively, because cells perform more divisions, which gives more percentage of offspring cells) at 21% oxygen. Compared to males, the female CD4+ cells showed increased susceptibility to apoptosis at both O₂ concentrations. No differences in the levels of cytokines regardless of gender and oxygen conditions were found.</p><p>CONCLUSIONS: We showed that in vitro female T cells (both CD4+ and CD8+ cells) are more sensitive than male lymphocytes to low O2 concentration as demonstrated by the decrease in their proliferation dynamics. The effect does not depend on increased apoptosis of female T cells under low O₂ because percentage of apoptotic cells was similar at both O₂ concentrations.</p>}},
author = {{Waskowska, Agnieszka and Lisowska, Katarzyna A and Daca, Agnieszka and Henc, Izabella and Brandberg, Fredrik and Mazurek, Paula and Brzustewicz, Edyta and Witkowski, Jacek M and Bryl, Ewa}},
issn = {{0239-8508}},
keywords = {{Adult; Apoptosis/drug effects; CD4-CD8 Ratio; Cell Proliferation/drug effects; Cells, Cultured; Female; Humans; Lymphocyte Activation/drug effects; Male; Oxygen/pharmacology; Sex Factors; T-Lymphocytes/cytology}},
language = {{eng}},
number = {{1}},
pages = {{26--36}},
publisher = {{Via Medica}},
series = {{Folia histochemica et cytobiologica}},
title = {{Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women}},
url = {{http://dx.doi.org/10.5603/FHC.a2017.0006}},
doi = {{10.5603/FHC.a2017.0006}},
volume = {{55}},
year = {{2017}},
}