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Neurochemical profile of the developing mouse cortex determined by in vivo 1H NMR spectroscopy at 14.1 T and the effect of recurrent anaesthesia

Kulak, Anita; Duarte, João M N LU ; Do, Kim Q and Gruetter, Rolf (2010) In Journal of Neurochemistry 115(6). p.1466-1477
Abstract

The neurochemical profile of the cortex develops in a region and time specific manner, which can be distorted by psychiatric and other neurological pathologies. Pre-clinical studies often involve experimental mouse models. In this study, we determined the neurochemical profile of C57BL/6 mice in a longitudinal study design to provide a reference frame for the normal developing mouse cortex. Using in vivo proton NMR spectroscopy at 14 T, we measured the concentrations of 18 metabolites in the anterior and posterior cortex on postnatal days (P) 10, 20, 30, 60 and 90. Cortical development was marked by alterations of highly concentrated metabolites, such as N-acetylaspartate, glutamate, taurine and creatine. Regional specificity was... (More)

The neurochemical profile of the cortex develops in a region and time specific manner, which can be distorted by psychiatric and other neurological pathologies. Pre-clinical studies often involve experimental mouse models. In this study, we determined the neurochemical profile of C57BL/6 mice in a longitudinal study design to provide a reference frame for the normal developing mouse cortex. Using in vivo proton NMR spectroscopy at 14 T, we measured the concentrations of 18 metabolites in the anterior and posterior cortex on postnatal days (P) 10, 20, 30, 60 and 90. Cortical development was marked by alterations of highly concentrated metabolites, such as N-acetylaspartate, glutamate, taurine and creatine. Regional specificity was represented by early variations in the concentration of glutamine, aspartate and choline. In adult animals, regional concentration differences were found for N-acetylaspartate, creatine and myo-inositol. In this study, animals were exposed to recurrent isoflurane anaesthesia. Additional experiments showed that the latter was devoid of major effects on behaviour or cortical neurochemical profile. In conclusion, the high sensitivity and reproducibility of the measurements achieved at 14 T allowed us to identify developmental variations of cortical areas within the mouse cortex.

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author
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Contribution to journal
publication status
published
keywords
Anesthesia, Inhalation, Animals, Animals, Newborn, Behavior, Animal, Cerebral Cortex, Energy Metabolism, Female, Isoflurane, Magnetic Resonance Spectroscopy, Male, Maze Learning, Mice, Mice, Inbred C57BL, Protons, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurochemistry
volume
115
issue
6
pages
12 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:78649988282
ISSN
1471-4159
DOI
10.1111/j.1471-4159.2010.07051.x
language
English
LU publication?
no
id
1e0a732a-e9ae-48ef-83f0-f8b0d1aa001a
date added to LUP
2017-10-19 15:11:59
date last changed
2018-07-01 04:50:06
@article{1e0a732a-e9ae-48ef-83f0-f8b0d1aa001a,
  abstract     = {<p>The neurochemical profile of the cortex develops in a region and time specific manner, which can be distorted by psychiatric and other neurological pathologies. Pre-clinical studies often involve experimental mouse models. In this study, we determined the neurochemical profile of C57BL/6 mice in a longitudinal study design to provide a reference frame for the normal developing mouse cortex. Using in vivo proton NMR spectroscopy at 14 T, we measured the concentrations of 18 metabolites in the anterior and posterior cortex on postnatal days (P) 10, 20, 30, 60 and 90. Cortical development was marked by alterations of highly concentrated metabolites, such as N-acetylaspartate, glutamate, taurine and creatine. Regional specificity was represented by early variations in the concentration of glutamine, aspartate and choline. In adult animals, regional concentration differences were found for N-acetylaspartate, creatine and myo-inositol. In this study, animals were exposed to recurrent isoflurane anaesthesia. Additional experiments showed that the latter was devoid of major effects on behaviour or cortical neurochemical profile. In conclusion, the high sensitivity and reproducibility of the measurements achieved at 14 T allowed us to identify developmental variations of cortical areas within the mouse cortex.</p>},
  author       = {Kulak, Anita and Duarte, João M N and Do, Kim Q and Gruetter, Rolf},
  issn         = {1471-4159},
  keyword      = {Anesthesia, Inhalation,Animals,Animals, Newborn,Behavior, Animal,Cerebral Cortex,Energy Metabolism,Female,Isoflurane,Magnetic Resonance Spectroscopy,Male,Maze Learning,Mice,Mice, Inbred C57BL,Protons,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {6},
  pages        = {1466--1477},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Neurochemical profile of the developing mouse cortex determined by in vivo 1H NMR spectroscopy at 14.1 T and the effect of recurrent anaesthesia},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.2010.07051.x},
  volume       = {115},
  year         = {2010},
}