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An efficient and versatile membraneless bioanode for biofuel cells based on Corynascus thermophilus cellobiose dehydrogenase

Zafar, Muhammad Nadeem; Aslam, Iqra; Ludwig, Roland; Xu, Guobao and Gorton, Lo LU (2019) In Electrochimica Acta 295. p.316-324
Abstract

In the present study, the effect of carbon nanotubes (CNTs) either single walled (SWCNT) or multi walled (MWCNT) on sugar oxidation by Corynascus thermophilus cellobiose dehydrogenase (CtCDH) is investigated. The current observed for the direct electron transfer (DET) communication between the enzyme and the electrode is compared to that of the mediated electron transfer (MET) obtained from CtCDH/CNT/Os-polymer modified electrodes. The CNTs provide a high surface area for the immobilization of CtCDH and the Os-polymer and increases the amount of both on the electrode surface. The amperometric current density measured for 50 mM glucose at pH 7.4 and an applied potential of 290 mV vs. SHE in DET mode is 6.90 μA cm−2, which is... (More)

In the present study, the effect of carbon nanotubes (CNTs) either single walled (SWCNT) or multi walled (MWCNT) on sugar oxidation by Corynascus thermophilus cellobiose dehydrogenase (CtCDH) is investigated. The current observed for the direct electron transfer (DET) communication between the enzyme and the electrode is compared to that of the mediated electron transfer (MET) obtained from CtCDH/CNT/Os-polymer modified electrodes. The CNTs provide a high surface area for the immobilization of CtCDH and the Os-polymer and increases the amount of both on the electrode surface. The amperometric current density measured for 50 mM glucose at pH 7.4 and an applied potential of 290 mV vs. SHE in DET mode is 6.90 μA cm−2, which is further improved to about 8.73 μA cm−2 when a cross-linker is applied, whereas a 14-folds increase to 97.6 μA cm−2 is observed for MET when CtCDH is immobilized in the Os-polymer and cross-linked. The current densities extracted from CVs at 150 mV vs. SHE using 20 mM glucose for CtCDH immobilized with the Os-polymer, MWCNT/Os-polymer and SWCNT/Os-polymer are 139.7, 180.3 and 328.2 μA cm−2, respectively so a remarkable increase is observed when using SWCNT for electrode modification. The bioanode also exhibits a good stability and it retains more than 50% of its activity in multicycle CV scan after five days. Thus the bioanode based on SWCNT/CtCDH/Os-polymer/cross-linker could be a promising electrode for application as anode in enzymatic biofuel cells.

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Contribution to journal
publication status
published
subject
keywords
Bioanode, Biofuel cell, Carbon nanotubes, Cellobiose dehydrogenase, Direct electron transfer, Glucose, Mediate electron transfer, Osmium polymer
in
Electrochimica Acta
volume
295
pages
9 pages
publisher
Pergamon
external identifiers
  • scopus:85055969335
ISSN
0013-4686
DOI
10.1016/j.electacta.2018.10.047
language
English
LU publication?
yes
id
1e23cb12-c59d-427c-a68d-66cc7eb155b7
date added to LUP
2018-11-14 08:06:11
date last changed
2019-10-15 06:50:14
@article{1e23cb12-c59d-427c-a68d-66cc7eb155b7,
  abstract     = {<p>In the present study, the effect of carbon nanotubes (CNTs) either single walled (SWCNT) or multi walled (MWCNT) on sugar oxidation by Corynascus thermophilus cellobiose dehydrogenase (CtCDH) is investigated. The current observed for the direct electron transfer (DET) communication between the enzyme and the electrode is compared to that of the mediated electron transfer (MET) obtained from CtCDH/CNT/Os-polymer modified electrodes. The CNTs provide a high surface area for the immobilization of CtCDH and the Os-polymer and increases the amount of both on the electrode surface. The amperometric current density measured for 50 mM glucose at pH 7.4 and an applied potential of 290 mV vs. SHE in DET mode is 6.90 μA cm<sup>−2</sup>, which is further improved to about 8.73 μA cm<sup>−2</sup> when a cross-linker is applied, whereas a 14-folds increase to 97.6 μA cm<sup>−2</sup> is observed for MET when CtCDH is immobilized in the Os-polymer and cross-linked. The current densities extracted from CVs at 150 mV vs. SHE using 20 mM glucose for CtCDH immobilized with the Os-polymer, MWCNT/Os-polymer and SWCNT/Os-polymer are 139.7, 180.3 and 328.2 μA cm<sup>−2</sup>, respectively so a remarkable increase is observed when using SWCNT for electrode modification. The bioanode also exhibits a good stability and it retains more than 50% of its activity in multicycle CV scan after five days. Thus the bioanode based on SWCNT/CtCDH/Os-polymer/cross-linker could be a promising electrode for application as anode in enzymatic biofuel cells.</p>},
  author       = {Zafar, Muhammad Nadeem and Aslam, Iqra and Ludwig, Roland and Xu, Guobao and Gorton, Lo},
  issn         = {0013-4686},
  keyword      = {Bioanode,Biofuel cell,Carbon nanotubes,Cellobiose dehydrogenase,Direct electron transfer,Glucose,Mediate electron transfer,Osmium polymer},
  language     = {eng},
  pages        = {316--324},
  publisher    = {Pergamon},
  series       = {Electrochimica Acta},
  title        = {An efficient and versatile membraneless bioanode for biofuel cells based on Corynascus thermophilus cellobiose dehydrogenase},
  url          = {http://dx.doi.org/10.1016/j.electacta.2018.10.047},
  volume       = {295},
  year         = {2019},
}