Total internal reflection fluorescence microscopy to study GLUT4 trafficking
(2018) In Methods in Molecular Biology 1713. p.151-159- Abstract
Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1e391747-8b6d-4530-987a-560022606ca3
- author
- Wasserstrom, Sebastian LU ; Morén, Björn LU and Stenkula, Karin G. LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Fluorescence, GLUT4, GLUT4 storage vesicles, GSV, Microscopy, Primary adipocytes, TIRF
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1713
- pages
- 9 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:29218524
- scopus:85037585365
- ISSN
- 1064-3745
- DOI
- 10.1007/978-1-4939-7507-5_12
- language
- English
- LU publication?
- yes
- id
- 1e391747-8b6d-4530-987a-560022606ca3
- date added to LUP
- 2017-12-21 08:29:39
- date last changed
- 2024-09-02 14:50:53
@inbook{1e391747-8b6d-4530-987a-560022606ca3, abstract = {{<p>Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.</p>}}, author = {{Wasserstrom, Sebastian and Morén, Björn and Stenkula, Karin G.}}, booktitle = {{Methods in Molecular Biology}}, issn = {{1064-3745}}, keywords = {{Fluorescence; GLUT4; GLUT4 storage vesicles; GSV; Microscopy; Primary adipocytes; TIRF}}, language = {{eng}}, pages = {{151--159}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Total internal reflection fluorescence microscopy to study GLUT4 trafficking}}, url = {{http://dx.doi.org/10.1007/978-1-4939-7507-5_12}}, doi = {{10.1007/978-1-4939-7507-5_12}}, volume = {{1713}}, year = {{2018}}, }