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Total internal reflection fluorescence microscopy to study GLUT4 trafficking

Wasserstrom, Sebastian LU ; Morén, Björn LU and Stenkula, Karin G. LU (2018) In Methods in Molecular Biology 1713. p.151-159
Abstract

Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Fluorescence, GLUT4, GLUT4 storage vesicles, GSV, Microscopy, Primary adipocytes, TIRF
in
Methods in Molecular Biology
volume
1713
pages
9 pages
publisher
Humana Press
external identifiers
  • scopus:85037585365
ISSN
1064-3745
DOI
10.1007/978-1-4939-7507-5_12
language
English
LU publication?
yes
id
1e391747-8b6d-4530-987a-560022606ca3
date added to LUP
2017-12-21 08:29:39
date last changed
2018-10-16 05:46:20
@inbook{1e391747-8b6d-4530-987a-560022606ca3,
  abstract     = {<p>Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes.</p>},
  author       = {Wasserstrom, Sebastian and Morén, Björn and Stenkula, Karin G.},
  issn         = {1064-3745},
  keyword      = {Fluorescence,GLUT4,GLUT4 storage vesicles,GSV,Microscopy,Primary adipocytes,TIRF},
  language     = {eng},
  pages        = {151--159},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Total internal reflection fluorescence microscopy to study GLUT4 trafficking},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7507-5_12},
  volume       = {1713},
  year         = {2018},
}