Advanced

The impact of deuteration on natural and synthetic lipids : A neutron diffraction study

Luchini, Alessandra; Delhom, Robin LU ; Demé, Bruno; Laux, Valérie; Moulin, Martine; Haertlein, Michael; Pichler, Harald; Strohmeier, Gernot A.; Wacklin, Hanna LU and Fragneto, Giovanna (2018) In Colloids and Surfaces B: Biointerfaces
Abstract

The structural investigation of cellular membranes requires access to model systems where the molecular complexity is representative of the cellular environment and that allow for the exploitation of structural techniques. Neutron scattering, and in particular neutron diffraction can provide unique and detailed information on the structure of lipid membranes. However, deuterated samples are desirable to fully exploit this powerful method. Recently, the extraction of lipids from microorganisms grown in deuterated media was demonstrated to be both an attracting route to obtain complex lipid mixtures resembling the composition of natural membranes, and to producing deuterated molecules in a very convenient way. A full characterization of... (More)

The structural investigation of cellular membranes requires access to model systems where the molecular complexity is representative of the cellular environment and that allow for the exploitation of structural techniques. Neutron scattering, and in particular neutron diffraction can provide unique and detailed information on the structure of lipid membranes. However, deuterated samples are desirable to fully exploit this powerful method. Recently, the extraction of lipids from microorganisms grown in deuterated media was demonstrated to be both an attracting route to obtain complex lipid mixtures resembling the composition of natural membranes, and to producing deuterated molecules in a very convenient way. A full characterization of these deuterated extracts is hence pivotal for their use in building up model membrane systems. Here we report the structural characterization of lipid extracts obtained from Pichia pastoris by means of neutron diffraction measurements. In particular, we compare the structure of membranes extracted from yeast cells grown in a standard culture medium and in a corresponding deuterated culture medium. The results show that the different molecular composition of the deuterated and protiated lipid extracts induce different structural organization of the lipid membranes. In addition, we compare these membranes composed of extracted yeast lipids with stacked bilayers prepared from synthetic lipid mixtures.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Cholesterol, Deuteration, Ergosterol, Neutron diffraction, Yeast lipids
in
Colloids and Surfaces B: Biointerfaces
publisher
Elsevier
external identifiers
  • scopus:85041555102
ISSN
0927-7765
DOI
10.1016/j.colsurfb.2018.02.009
language
English
LU publication?
yes
id
1e78ed02-faf1-4e18-a58c-dfc4fbb5b84d
date added to LUP
2018-02-22 07:57:27
date last changed
2018-05-29 10:03:37
@article{1e78ed02-faf1-4e18-a58c-dfc4fbb5b84d,
  abstract     = {<p>The structural investigation of cellular membranes requires access to model systems where the molecular complexity is representative of the cellular environment and that allow for the exploitation of structural techniques. Neutron scattering, and in particular neutron diffraction can provide unique and detailed information on the structure of lipid membranes. However, deuterated samples are desirable to fully exploit this powerful method. Recently, the extraction of lipids from microorganisms grown in deuterated media was demonstrated to be both an attracting route to obtain complex lipid mixtures resembling the composition of natural membranes, and to producing deuterated molecules in a very convenient way. A full characterization of these deuterated extracts is hence pivotal for their use in building up model membrane systems. Here we report the structural characterization of lipid extracts obtained from Pichia pastoris by means of neutron diffraction measurements. In particular, we compare the structure of membranes extracted from yeast cells grown in a standard culture medium and in a corresponding deuterated culture medium. The results show that the different molecular composition of the deuterated and protiated lipid extracts induce different structural organization of the lipid membranes. In addition, we compare these membranes composed of extracted yeast lipids with stacked bilayers prepared from synthetic lipid mixtures.</p>},
  author       = {Luchini, Alessandra and Delhom, Robin and Demé, Bruno and Laux, Valérie and Moulin, Martine and Haertlein, Michael and Pichler, Harald and Strohmeier, Gernot A. and Wacklin, Hanna and Fragneto, Giovanna},
  issn         = {0927-7765},
  keyword      = {Cholesterol,Deuteration,Ergosterol,Neutron diffraction,Yeast lipids},
  language     = {eng},
  month        = {02},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {The impact of deuteration on natural and synthetic lipids : A neutron diffraction study},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2018.02.009},
  year         = {2018},
}