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Structural and Functional Analysis of the ApolipoproteinA-I A164S Variant.

Dalla-Riva, Jonathan LU ; Lagerstedt, Jens LU and Petrlova, Jitka LU (2015) In PLoS ONE 10(11).
Abstract
Apolipoprotein A-I (apoA-I) is the main protein involved in the formation of high-density lipoprotein (HDL), it is the principal mediator of the reverse cholesterol transfer (RCT) pathway and provides cardio-protection. In addition to functional wild-type apoA-I, several variants have been shown to associate with hereditary amyloidosis. In this study we have performed biophysical and biochemical analyses of the structure and functional properties of the A164S variant of apoA-I (1:500 in the Danish general population), which is the first known mutation of apoA-I that leads to an increased risk of ischaemic heart disease (IHD), myocardial infarction and mortality without associated low HDL cholesterol levels. Despite the fact that... (More)
Apolipoprotein A-I (apoA-I) is the main protein involved in the formation of high-density lipoprotein (HDL), it is the principal mediator of the reverse cholesterol transfer (RCT) pathway and provides cardio-protection. In addition to functional wild-type apoA-I, several variants have been shown to associate with hereditary amyloidosis. In this study we have performed biophysical and biochemical analyses of the structure and functional properties of the A164S variant of apoA-I (1:500 in the Danish general population), which is the first known mutation of apoA-I that leads to an increased risk of ischaemic heart disease (IHD), myocardial infarction and mortality without associated low HDL cholesterol levels. Despite the fact that epidemiologically IHD is associated with low plasma levels of HDL, the A164S mutation is linked to normal plasma levels of lipids, HDL and apoA-I, suggesting impaired functionality of this variant. Using biophysical techniques (e.g., circular dichroism spectroscopy and electron microscopy) to determine secondary structure, stability and pro-amyloidogenic property of the lipid free A164S apoA-I variant, our observations suggest similarity in structural properties between apoA-I WT and apoA-I A164S. However, the A164S apoA-I variant exhibits lower binding affinity to lipids but forms similar sized HDL particles to those produced by WT. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
10
issue
11
article number
e0143915
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:26605794
  • wos:000365865300123
  • scopus:84955594337
  • pmid:26605794
ISSN
1932-6203
DOI
10.1371/journal.pone.0143915
language
English
LU publication?
yes
id
1f7fd750-207f-43ba-9eeb-07c4d165d8da (old id 8234548)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26605794?dopt=Abstract
date added to LUP
2016-04-01 15:02:44
date last changed
2022-04-06 21:57:33
@article{1f7fd750-207f-43ba-9eeb-07c4d165d8da,
  abstract     = {{Apolipoprotein A-I (apoA-I) is the main protein involved in the formation of high-density lipoprotein (HDL), it is the principal mediator of the reverse cholesterol transfer (RCT) pathway and provides cardio-protection. In addition to functional wild-type apoA-I, several variants have been shown to associate with hereditary amyloidosis. In this study we have performed biophysical and biochemical analyses of the structure and functional properties of the A164S variant of apoA-I (1:500 in the Danish general population), which is the first known mutation of apoA-I that leads to an increased risk of ischaemic heart disease (IHD), myocardial infarction and mortality without associated low HDL cholesterol levels. Despite the fact that epidemiologically IHD is associated with low plasma levels of HDL, the A164S mutation is linked to normal plasma levels of lipids, HDL and apoA-I, suggesting impaired functionality of this variant. Using biophysical techniques (e.g., circular dichroism spectroscopy and electron microscopy) to determine secondary structure, stability and pro-amyloidogenic property of the lipid free A164S apoA-I variant, our observations suggest similarity in structural properties between apoA-I WT and apoA-I A164S. However, the A164S apoA-I variant exhibits lower binding affinity to lipids but forms similar sized HDL particles to those produced by WT.}},
  author       = {{Dalla-Riva, Jonathan and Lagerstedt, Jens and Petrlova, Jitka}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  number       = {{11}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Structural and Functional Analysis of the ApolipoproteinA-I A164S Variant.}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0143915}},
  doi          = {{10.1371/journal.pone.0143915}},
  volume       = {{10}},
  year         = {{2015}},
}