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Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

Heuer, Andreas LU (2021) In Methods in Molecular Biology 2352. p.201-226
Abstract

The detection of neurotransmitter release from reprogrammed human cell is an important demonstration of their functionality. Electrochemistry has the distinct advantages over alternative methods that it allows for the measuring of the analyte of interest at a high temporal resolution. This is necessary for fast events, such as neurotransmitter release and reuptake, which happen in the order of milliseconds to seconds. The precise description of these kinetic events can lead to insights into the function of cells in health and disease and allows for the exploration of events that might be missed using methods that look at absolute concentration values or methods that have a slower sampling rate. In the present chapter, we describe the... (More)

The detection of neurotransmitter release from reprogrammed human cell is an important demonstration of their functionality. Electrochemistry has the distinct advantages over alternative methods that it allows for the measuring of the analyte of interest at a high temporal resolution. This is necessary for fast events, such as neurotransmitter release and reuptake, which happen in the order of milliseconds to seconds. The precise description of these kinetic events can lead to insights into the function of cells in health and disease and allows for the exploration of events that might be missed using methods that look at absolute concentration values or methods that have a slower sampling rate. In the present chapter, we describe the use of constant potential amperometry and enzyme-coated multielectrode arrays for the detection of glutamate in vitro. These biosensors have the distinct advantage of “self-referencing,” a method providing high selectivity while retaining outstanding temporal resolution. Here, we provide a step-by-step user guide for a commercially available system and its application for in vitro systems such as reprogrammed cells.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Amperometry, Astrocytes, Biosensor, Electrochemistry, Enzyme-based, Glutamate, In vitro, MEA, Neurotransmitter reuptake, Reprogramming, Stem cells
host publication
Neural Reprogramming : Methods and Protocols - Methods and Protocols
series title
Methods in Molecular Biology
volume
2352
pages
26 pages
publisher
Humana Press
external identifiers
  • pmid:34324189
  • scopus:85111893395
ISSN
1940-6029
1064-3745
ISBN
978-1-0716-1601-7
978-1-0716-1600-0
DOI
10.1007/978-1-0716-1601-7_14
language
English
LU publication?
yes
id
1f95a951-90a8-4386-bd39-998c4642b895
date added to LUP
2021-12-10 09:33:24
date last changed
2022-05-05 06:33:51
@inbook{1f95a951-90a8-4386-bd39-998c4642b895,
  abstract     = {{<p>The detection of neurotransmitter release from reprogrammed human cell is an important demonstration of their functionality. Electrochemistry has the distinct advantages over alternative methods that it allows for the measuring of the analyte of interest at a high temporal resolution. This is necessary for fast events, such as neurotransmitter release and reuptake, which happen in the order of milliseconds to seconds. The precise description of these kinetic events can lead to insights into the function of cells in health and disease and allows for the exploration of events that might be missed using methods that look at absolute concentration values or methods that have a slower sampling rate. In the present chapter, we describe the use of constant potential amperometry and enzyme-coated multielectrode arrays for the detection of glutamate in vitro. These biosensors have the distinct advantage of “self-referencing,” a method providing high selectivity while retaining outstanding temporal resolution. Here, we provide a step-by-step user guide for a commercially available system and its application for in vitro systems such as reprogrammed cells.</p>}},
  author       = {{Heuer, Andreas}},
  booktitle    = {{Neural Reprogramming : Methods and Protocols}},
  isbn         = {{978-1-0716-1601-7}},
  issn         = {{1940-6029}},
  keywords     = {{Amperometry; Astrocytes; Biosensor; Electrochemistry; Enzyme-based; Glutamate; In vitro; MEA; Neurotransmitter reuptake; Reprogramming; Stem cells}},
  language     = {{eng}},
  pages        = {{201--226}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-1601-7_14}},
  doi          = {{10.1007/978-1-0716-1601-7_14}},
  volume       = {{2352}},
  year         = {{2021}},
}