Automated high-performance liquid chromatographic determination of 5-S-cysteinyl-3,4-dihydroxyphenylalanine in urine
(1989) In Journal of Chromatography A 473. p.359-370- Abstract
- An automated high-performance liquid chromatographic (HPLC) method has been developed for measurement of 5-S-cysteinyl-DOPA in urine (DOPA = 3,4-dihydroxyphenylalanine). The urinary sample was injected into an HPLC boronate column. With a mobile phase of 0.1 M phosphate buffer containing 0.2 mM disodium ethylenediaminetetraacetate (Na2EDTA) (pH 6.0) mixed with methanol (9:1), 5-S-cysteinyl-DOPA was adsorbed while most other compounds were washed away. By column switching, the column flow was reversed and 5-S-cysteinyl-DOPA was desorbed by a mobile phase of 0.1 M formic acid and 0.2 mM Na2EDTA at pH 3.0 and chromatographed on a reversed-phase column. The precision, as estimated from repeated analysis of an urinary sample and from duplicate... (More)
- An automated high-performance liquid chromatographic (HPLC) method has been developed for measurement of 5-S-cysteinyl-DOPA in urine (DOPA = 3,4-dihydroxyphenylalanine). The urinary sample was injected into an HPLC boronate column. With a mobile phase of 0.1 M phosphate buffer containing 0.2 mM disodium ethylenediaminetetraacetate (Na2EDTA) (pH 6.0) mixed with methanol (9:1), 5-S-cysteinyl-DOPA was adsorbed while most other compounds were washed away. By column switching, the column flow was reversed and 5-S-cysteinyl-DOPA was desorbed by a mobile phase of 0.1 M formic acid and 0.2 mM Na2EDTA at pH 3.0 and chromatographed on a reversed-phase column. The precision, as estimated from repeated analysis of an urinary sample and from duplicate analysis of a number of samples, ranged from 1.4 to 5.2% (coefficient of variation), and the analytical recovery was 93 +/- 4.1%. The method is suitable for use in the clinical laboratory. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1104709
- author
- Kagedal, Bertil ; Källberg, Magnus ; Årstrand, Kerstin and Hansson, Christer LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 473
- pages
- 359 - 370
- publisher
- Elsevier
- external identifiers
-
- pmid:2504759
- scopus:0024364327
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(00)91320-7
- language
- English
- LU publication?
- yes
- id
- 1f9dc49a-d43e-41fb-ae7b-bcfd150ae31f (old id 1104709)
- date added to LUP
- 2016-04-01 16:35:01
- date last changed
- 2021-01-03 09:56:59
@article{1f9dc49a-d43e-41fb-ae7b-bcfd150ae31f,
abstract = {{An automated high-performance liquid chromatographic (HPLC) method has been developed for measurement of 5-S-cysteinyl-DOPA in urine (DOPA = 3,4-dihydroxyphenylalanine). The urinary sample was injected into an HPLC boronate column. With a mobile phase of 0.1 M phosphate buffer containing 0.2 mM disodium ethylenediaminetetraacetate (Na2EDTA) (pH 6.0) mixed with methanol (9:1), 5-S-cysteinyl-DOPA was adsorbed while most other compounds were washed away. By column switching, the column flow was reversed and 5-S-cysteinyl-DOPA was desorbed by a mobile phase of 0.1 M formic acid and 0.2 mM Na2EDTA at pH 3.0 and chromatographed on a reversed-phase column. The precision, as estimated from repeated analysis of an urinary sample and from duplicate analysis of a number of samples, ranged from 1.4 to 5.2% (coefficient of variation), and the analytical recovery was 93 +/- 4.1%. The method is suitable for use in the clinical laboratory.}},
author = {{Kagedal, Bertil and Källberg, Magnus and Årstrand, Kerstin and Hansson, Christer}},
issn = {{0021-9673}},
language = {{eng}},
pages = {{359--370}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography A}},
title = {{Automated high-performance liquid chromatographic determination of 5-S-cysteinyl-3,4-dihydroxyphenylalanine in urine}},
url = {{http://dx.doi.org/10.1016/S0021-9673(00)91320-7}},
doi = {{10.1016/S0021-9673(00)91320-7}},
volume = {{473}},
year = {{1989}},
}