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Scanning electron microscopy of surface changes on dispersed pancreatic β-cells following stimulation of insulin release

Lernmark, A. LU orcid and Winblad, B. (1977) In Medical Biology 55(3). p.141-147
Abstract

The surface structure of isolated viable β-cells was studied by scanning electron microscopy. Suspensions of islet cells were prepared from the β-cell-rich islets of the ob/ob mouse. Cells incubated with and without D-glucose were fixed while in suspensions, filtered onto Nucleopore filters and prepared for scanning electron microscopy by the critical point drying procedure. After incubation in glucose free medium the cells were spherical with surface blebs and occasional small pits. Cells stimulated with 20 mM D-glucose and 0.1 mM 3-isobutyl-1-methyl-xanthine exhibited a two fold increase in the number of blebs and in insulin release. The increased number of blebs was still seen in a Ca++ deficient medium, and may,... (More)

The surface structure of isolated viable β-cells was studied by scanning electron microscopy. Suspensions of islet cells were prepared from the β-cell-rich islets of the ob/ob mouse. Cells incubated with and without D-glucose were fixed while in suspensions, filtered onto Nucleopore filters and prepared for scanning electron microscopy by the critical point drying procedure. After incubation in glucose free medium the cells were spherical with surface blebs and occasional small pits. Cells stimulated with 20 mM D-glucose and 0.1 mM 3-isobutyl-1-methyl-xanthine exhibited a two fold increase in the number of blebs and in insulin release. The increased number of blebs was still seen in a Ca++ deficient medium, and may, therefore, not necessarily reflect an insulin extrusion phenomenon. It is concluded that D-glucose induces alternations of the surface morphology in isolated β-cells in suspension.

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author
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publishing date
type
Contribution to journal
publication status
published
in
Medical Biology
volume
55
issue
3
pages
7 pages
publisher
Taylor & Francis
external identifiers
  • pmid:330967
  • scopus:0017722909
ISSN
0302-2137
language
English
LU publication?
no
id
1fba535f-aceb-4613-bf82-8654ea131494
date added to LUP
2019-09-18 12:09:10
date last changed
2024-03-13 08:06:10
@article{1fba535f-aceb-4613-bf82-8654ea131494,
  abstract     = {{<p>The surface structure of isolated viable β-cells was studied by scanning electron microscopy. Suspensions of islet cells were prepared from the β-cell-rich islets of the ob/ob mouse. Cells incubated with and without D-glucose were fixed while in suspensions, filtered onto Nucleopore filters and prepared for scanning electron microscopy by the critical point drying procedure. After incubation in glucose free medium the cells were spherical with surface blebs and occasional small pits. Cells stimulated with 20 mM D-glucose and 0.1 mM 3-isobutyl-1-methyl-xanthine exhibited a two fold increase in the number of blebs and in insulin release. The increased number of blebs was still seen in a Ca<sup>++</sup> deficient medium, and may, therefore, not necessarily reflect an insulin extrusion phenomenon. It is concluded that D-glucose induces alternations of the surface morphology in isolated β-cells in suspension.</p>}},
  author       = {{Lernmark, A. and Winblad, B.}},
  issn         = {{0302-2137}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{3}},
  pages        = {{141--147}},
  publisher    = {{Taylor & Francis}},
  series       = {{Medical Biology}},
  title        = {{Scanning electron microscopy of surface changes on dispersed pancreatic β-cells following stimulation of insulin release}},
  volume       = {{55}},
  year         = {{1977}},
}