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Use of molecularly imprinted polymers in a biotransformation process

Ye, Lei LU orcid ; Ramström, Olof ; Ansell, Richard J. ; Månsson, Mats Olle LU and Mosbach, Klaus LU (1999) In Biotechnology and Bioengineering 64(6). p.650-655
Abstract

Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to give Z-L-Asp-L-Phe-OMe (Z- aspartame) was chosen as a model system to evaluate the applicability of using molecularly imprinted polymers to facilitate product formation. When the product-imprinted polymer is present, a considerable increase (40%) in product yield is obtained. Besides their use to enhance product yields, as demonstrated here, we suggest that imprinted polymers may also find use in the continuous... (More)

Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to give Z-L-Asp-L-Phe-OMe (Z- aspartame) was chosen as a model system to evaluate the applicability of using molecularly imprinted polymers to facilitate product formation. When the product-imprinted polymer is present, a considerable increase (40%) in product yield is obtained. Besides their use to enhance product yields, as demonstrated here, we suggest that imprinted polymers may also find use in the continuous removal of toxic compounds during biochemical reactions.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aspartame, Enzymatic synthesis, Molecular imprinting, Specific adsorbents, Thermolysin
in
Biotechnology and Bioengineering
volume
64
issue
6
pages
6 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0033588808
  • pmid:10417213
ISSN
0006-3592
DOI
10.1002/(SICI)1097-0290(19990920)64:6<650::AID-BIT3>3.0.CO;2-7
language
English
LU publication?
yes
id
1fbb6935-03f4-468c-8093-3ff74c2eebd8
date added to LUP
2019-06-18 09:04:35
date last changed
2025-04-04 14:10:14
@article{1fbb6935-03f4-468c-8093-3ff74c2eebd8,
  abstract     = {{<p>Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to give Z-L-Asp-L-Phe-OMe (Z- aspartame) was chosen as a model system to evaluate the applicability of using molecularly imprinted polymers to facilitate product formation. When the product-imprinted polymer is present, a considerable increase (40%) in product yield is obtained. Besides their use to enhance product yields, as demonstrated here, we suggest that imprinted polymers may also find use in the continuous removal of toxic compounds during biochemical reactions.</p>}},
  author       = {{Ye, Lei and Ramström, Olof and Ansell, Richard J. and Månsson, Mats Olle and Mosbach, Klaus}},
  issn         = {{0006-3592}},
  keywords     = {{Aspartame; Enzymatic synthesis; Molecular imprinting; Specific adsorbents; Thermolysin}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{6}},
  pages        = {{650--655}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Use of molecularly imprinted polymers in a biotransformation process}},
  url          = {{http://dx.doi.org/10.1002/(SICI)1097-0290(19990920)64:6<650::AID-BIT3>3.0.CO;2-7}},
  doi          = {{10.1002/(SICI)1097-0290(19990920)64:6<650::AID-BIT3>3.0.CO;2-7}},
  volume       = {{64}},
  year         = {{1999}},
}