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Evaluation of a tape-stripping technique for measuring dermal exposure to pyrene and benzo(a)pyrene.

Kammer, Ronny LU ; Tinnerberg, Håkan LU and Eriksson, Kåre (2011) In Journal of Environmental Monitoring 13(8). p.2165-2171
Abstract
Epidemiological studies have shown an increased incidence of cancer among workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs). As the dermal route is considered important for exposure to PAHs in the workplace, the aim of this study was to develop and evaluate a tape-stripping technique for monitoring dermal exposure to pyrene and benzo(a)pyrene. The tape-stripping method was evaluated by applying different concentrations of pyrene and benzo(a)pyrene directly onto tape strips (spiked tapes), clean glass plates, and on the skin of five volunteers. The glass plates were stripped using a single strip of tape and the skin of the volunteers was stripped with five consecutive strips of tape after 0 and 30 minutes. The method... (More)
Epidemiological studies have shown an increased incidence of cancer among workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs). As the dermal route is considered important for exposure to PAHs in the workplace, the aim of this study was to develop and evaluate a tape-stripping technique for monitoring dermal exposure to pyrene and benzo(a)pyrene. The tape-stripping method was evaluated by applying different concentrations of pyrene and benzo(a)pyrene directly onto tape strips (spiked tapes), clean glass plates, and on the skin of five volunteers. The glass plates were stripped using a single strip of tape and the skin of the volunteers was stripped with five consecutive strips of tape after 0 and 30 minutes. The method was also tested on five chimney sweeps at three exposure sites. High-performance liquid chromatography with fluorescence detection was employed for the quantification of pyrene and benzo(a)pyrene. The mean recovery from the spiked tapes was 97% for pyrene and 93% for benzo(a)pyrene. The mean overall recovery from the glass plates was 88% and 76% for pyrene and 88 and 85% for benzo(a)pyrene. The recovery from human skin was 70% and 63% for pyrene and 60 and 54% for benzo(a)pyrene, after 0 and 30 minutes, respectively. A concentration gradient was clearly detected between the five consecutive strips. Detectable amounts of pyrene and benzo(a)pyrene were found on all chimney sweeps at all exposure sites. This method can thus be used to detect and quantify dermal exposure to pyrene and benzo(a)pyrene. The results also show that pyrene and benzo(a)pyrene may be taken up by the skin. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Environmental Monitoring
volume
13
issue
8
pages
2165 - 2171
publisher
Royal Society of Chemistry
external identifiers
  • wos:000293502600008
  • pmid:21687840
  • scopus:79961151707
ISSN
1464-0325
DOI
10.1039/c1em10245a
language
English
LU publication?
yes
id
2b106941-715a-45f7-aa47-e39375218bd4 (old id 2007921)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21687840?dopt=Abstract
http://pubs.rsc.org/en/Content/ArticleLanding/2011/EM/c1em10245a
date added to LUP
2011-07-01 10:56:23
date last changed
2017-04-23 04:34:53
@article{2b106941-715a-45f7-aa47-e39375218bd4,
  abstract     = {Epidemiological studies have shown an increased incidence of cancer among workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs). As the dermal route is considered important for exposure to PAHs in the workplace, the aim of this study was to develop and evaluate a tape-stripping technique for monitoring dermal exposure to pyrene and benzo(a)pyrene. The tape-stripping method was evaluated by applying different concentrations of pyrene and benzo(a)pyrene directly onto tape strips (spiked tapes), clean glass plates, and on the skin of five volunteers. The glass plates were stripped using a single strip of tape and the skin of the volunteers was stripped with five consecutive strips of tape after 0 and 30 minutes. The method was also tested on five chimney sweeps at three exposure sites. High-performance liquid chromatography with fluorescence detection was employed for the quantification of pyrene and benzo(a)pyrene. The mean recovery from the spiked tapes was 97% for pyrene and 93% for benzo(a)pyrene. The mean overall recovery from the glass plates was 88% and 76% for pyrene and 88 and 85% for benzo(a)pyrene. The recovery from human skin was 70% and 63% for pyrene and 60 and 54% for benzo(a)pyrene, after 0 and 30 minutes, respectively. A concentration gradient was clearly detected between the five consecutive strips. Detectable amounts of pyrene and benzo(a)pyrene were found on all chimney sweeps at all exposure sites. This method can thus be used to detect and quantify dermal exposure to pyrene and benzo(a)pyrene. The results also show that pyrene and benzo(a)pyrene may be taken up by the skin.},
  author       = {Kammer, Ronny and Tinnerberg, Håkan and Eriksson, Kåre},
  issn         = {1464-0325},
  language     = {eng},
  number       = {8},
  pages        = {2165--2171},
  publisher    = {Royal Society of Chemistry},
  series       = {Journal of Environmental Monitoring},
  title        = {Evaluation of a tape-stripping technique for measuring dermal exposure to pyrene and benzo(a)pyrene.},
  url          = {http://dx.doi.org/10.1039/c1em10245a},
  volume       = {13},
  year         = {2011},
}