Analysis of early-flowering genes at barley chromosome 2H expands the repertoire of mutant alleles at the Mat-c locus
(2020) In Plant Cell Reports 39(1). p.47-61- Abstract
Key message: Analyses of barley mat-c loss of function mutants reveal deletions, splice-site mutations and nonsynonymous substitutions in a key gene regulating early flowering. Abstract: Optimal timing of flowering is critical for reproductive success and crop yield improvement. Several major quantitative trait loci for flowering time variation have been identified in barley. In the present study, we analyzed two near-isogenic lines, BW507 and BW508, which were reported to carry two independent early-flowering mutant loci, mat-b.7 and mat-c.19, respectively. Both introgression segments are co-localized in the pericentromeric region of chromosome 2H. We mapped the mutation in BW507 to a 31 Mbp interval on chromosome 2HL and concluded... (More)
Key message: Analyses of barley mat-c loss of function mutants reveal deletions, splice-site mutations and nonsynonymous substitutions in a key gene regulating early flowering. Abstract: Optimal timing of flowering is critical for reproductive success and crop yield improvement. Several major quantitative trait loci for flowering time variation have been identified in barley. In the present study, we analyzed two near-isogenic lines, BW507 and BW508, which were reported to carry two independent early-flowering mutant loci, mat-b.7 and mat-c.19, respectively. Both introgression segments are co-localized in the pericentromeric region of chromosome 2H. We mapped the mutation in BW507 to a 31 Mbp interval on chromosome 2HL and concluded that BW507 has a deletion of Mat-c, which is an ortholog of Antirrhinum majus CENTRORADIALIS (AmCEN) and Arabidopsis thaliana TERMINAL FLOWER1 (AtTFL1). Contrary to previous reports, our data showed that both BW507 and BW508 are Mat-c deficient and none of them are mat-b.7 derived. This work complements previous studies by identifying the uncharacterized mat-c.19 mutant and seven additional mat-c mutants. Moreover, we explored the X-ray structure of AtTFL1 for prediction of the functional effects of nonsynonymous substitutions caused by mutations in Mat-c.
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- author
- Matyszczak, Izabela ; Tominska, Marta ; Zakhrabekova, Shakhira LU ; Dockter, Christoph and Hansson, Mats LU
- organization
- publishing date
- 2020-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CENTRORADIALIS, Earliness, Hordeum vulgare, Mat-b, TERMINAL FLOWER1, TFL1
- in
- Plant Cell Reports
- volume
- 39
- issue
- 1
- pages
- 15 pages
- publisher
- Springer
- external identifiers
-
- pmid:31541262
- scopus:85074040459
- ISSN
- 0721-7714
- DOI
- 10.1007/s00299-019-02472-4
- project
- Exploring barley mat-b mutants to identify an early maturity gene
- language
- English
- LU publication?
- yes
- id
- 201dc98a-3d92-4369-a4b8-195cea58f882
- date added to LUP
- 2019-11-07 10:57:07
- date last changed
- 2024-04-02 19:57:40
@article{201dc98a-3d92-4369-a4b8-195cea58f882,
abstract = {{<p>Key message: Analyses of barley mat-c loss of function mutants reveal deletions, splice-site mutations and nonsynonymous substitutions in a key gene regulating early flowering. Abstract: Optimal timing of flowering is critical for reproductive success and crop yield improvement. Several major quantitative trait loci for flowering time variation have been identified in barley. In the present study, we analyzed two near-isogenic lines, BW507 and BW508, which were reported to carry two independent early-flowering mutant loci, mat-b.7 and mat-c.19, respectively. Both introgression segments are co-localized in the pericentromeric region of chromosome 2H. We mapped the mutation in BW507 to a 31 Mbp interval on chromosome 2HL and concluded that BW507 has a deletion of Mat-c, which is an ortholog of Antirrhinum majus CENTRORADIALIS (AmCEN) and Arabidopsis thaliana TERMINAL FLOWER1 (AtTFL1). Contrary to previous reports, our data showed that both BW507 and BW508 are Mat-c deficient and none of them are mat-b.7 derived. This work complements previous studies by identifying the uncharacterized mat-c.19 mutant and seven additional mat-c mutants. Moreover, we explored the X-ray structure of AtTFL1 for prediction of the functional effects of nonsynonymous substitutions caused by mutations in Mat-c.</p>}},
author = {{Matyszczak, Izabela and Tominska, Marta and Zakhrabekova, Shakhira and Dockter, Christoph and Hansson, Mats}},
issn = {{0721-7714}},
keywords = {{CENTRORADIALIS; Earliness; Hordeum vulgare; Mat-b; TERMINAL FLOWER1; TFL1}},
language = {{eng}},
number = {{1}},
pages = {{47--61}},
publisher = {{Springer}},
series = {{Plant Cell Reports}},
title = {{Analysis of early-flowering genes at barley chromosome 2H expands the repertoire of mutant alleles at the Mat-c locus}},
url = {{http://dx.doi.org/10.1007/s00299-019-02472-4}},
doi = {{10.1007/s00299-019-02472-4}},
volume = {{39}},
year = {{2020}},
}