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Downstream Processing Using Membrane Technology

Persson, Anna LU (2001) In Department of Chemical Engineering 1, Lund University, Sweden 2001:1003.
Abstract (Swedish)
Popular Abstract in Swedish

Membranteknik är en separationsteknik där stora partiklar skiljs av från mindre partiklar m.h.a. ett membranfilter. I min forskning har jag tittat på hur membranfiltrering kan användas inom bioteknikindustrin. Jag har tittat på hur mjölksyra kan renas upp från en lösning av jäst "vetemjölsvälling" samt hur första steget i uppreningen av protein A från en industriellt producerad fermenteringslösning på bästa sätt kan designas med membranteknik. Tekniker som jag använt är mikrofiltrering, ultrafiltrering och elektrodialys. Mikrofiltrereing har jag använt för att separera mikroorganismer från lösningen. Med ultrafiltrering har jag avlägsnat stora molekyler så som proteiner från lösningen. M.h.a.... (More)
Popular Abstract in Swedish

Membranteknik är en separationsteknik där stora partiklar skiljs av från mindre partiklar m.h.a. ett membranfilter. I min forskning har jag tittat på hur membranfiltrering kan användas inom bioteknikindustrin. Jag har tittat på hur mjölksyra kan renas upp från en lösning av jäst "vetemjölsvälling" samt hur första steget i uppreningen av protein A från en industriellt producerad fermenteringslösning på bästa sätt kan designas med membranteknik. Tekniker som jag använt är mikrofiltrering, ultrafiltrering och elektrodialys. Mikrofiltrereing har jag använt för att separera mikroorganismer från lösningen. Med ultrafiltrering har jag avlägsnat stora molekyler så som proteiner från lösningen. M.h.a. elektrodialys har jag separerat laddade molekyler från oladdade molekyler samt omvandlat saltet natrium-laktat (NaLa) till mjölksyra (HLa). Mjölksyra kan användas för produktion av plast att användas till förpackningsmaterial och suturer (de trådar som operationsläkaren syr ihop dig med efter operationen). Fördelen med mjölksyraplast är att den är lättnedbrytbar samt att nedbrytningsprodukterna inte är farliga för miljön. Protein A används inom bioteknikindustrin för tillverkning av materiel till andra separationsmetoder som t.ex. kromatografi. (Less)
Abstract
Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat... (More)
Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat starch. In this process microfiltration and ultrafiltration are used to remove micro-organisms and proteins from the broth. Finally, water-splitting electrodialysis is used to convert the sodium lactate produced during the fermentation to lactic acid and sodium hydroxide. The use of sub-critical flux when separating micro-organisms from lactic-acid-containing fermentation broth has also been studied. Part of the work deals with the retention of proteins in microfiltration membranes and how this is affected by pH, ion strength and membrane material. Two different proteins, bovine serum albumin and protein A have been studied. (Less)
Please use this url to cite or link to this publication:
author
opponent
  • Prof Bowen, Richard, Uiniversity of Wales, Swansea
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Kemiteknik och kemisk teknologi, Biochemical technology, Chemical technology and engineering, Protein A, Bovine serum albumin, Lactic acid, Electrodialysis, Ultrafiltration, Microfiltration, Downstream processing, Membrane, Biokemisk teknik
in
Department of Chemical Engineering 1, Lund University, Sweden
volume
2001:1003
pages
156 pages
publisher
Department of Chemical Engineering, Lund University
defense location
Room B at The Center for Chemistry and Chemical Engineering (KC)
defense date
2001-09-14 13:15
ISSN
1100-2778
ISBN
91-628-4875-5
language
English
LU publication?
yes
id
788b475d-8304-4bb1-a3e0-746703438c16 (old id 20235)
date added to LUP
2007-05-28 08:37:16
date last changed
2016-09-19 08:44:58
@phdthesis{788b475d-8304-4bb1-a3e0-746703438c16,
  abstract     = {Membrane filtration is a very gentle separation technique and can be used at low temperatures. This makes it suitable for liquids that contain micro-organisms and/or are heat sensitive, which is often the case in liquids processed in the field of biotechnology. However, one drawback is that these liquids are often very complex, which is a disadvantage when using membrane technology as it makes the process difficult to optimise regarding both process parameters and cleaning. This thesis deals with different aspects of membrane filtration and how membranes can be applied in different stages of the downstream processing. The work presented in this thesis covers downstream processing in the production of lactic acid from fermented wheat starch. In this process microfiltration and ultrafiltration are used to remove micro-organisms and proteins from the broth. Finally, water-splitting electrodialysis is used to convert the sodium lactate produced during the fermentation to lactic acid and sodium hydroxide. The use of sub-critical flux when separating micro-organisms from lactic-acid-containing fermentation broth has also been studied. Part of the work deals with the retention of proteins in microfiltration membranes and how this is affected by pH, ion strength and membrane material. Two different proteins, bovine serum albumin and protein A have been studied.},
  author       = {Persson, Anna},
  isbn         = {91-628-4875-5},
  issn         = {1100-2778},
  keyword      = {Kemiteknik och kemisk teknologi,Biochemical technology,Chemical technology and engineering,Protein A,Bovine serum albumin,Lactic acid,Electrodialysis,Ultrafiltration,Microfiltration,Downstream processing,Membrane,Biokemisk teknik},
  language     = {eng},
  pages        = {156},
  publisher    = {Department of Chemical Engineering, Lund University},
  school       = {Lund University},
  series       = {Department of Chemical Engineering 1, Lund University, Sweden},
  title        = {Downstream Processing Using Membrane Technology},
  volume       = {2001:1003},
  year         = {2001},
}