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Locus-specific protocol for nine different innate immune genes (antimicrobial peptides: beta-defensins) across passerine bird species reveals within-species coding variation and a case of trans-species polymorphisms

Hellgren, Olof LU and Sheldon, B. C. (2011) In Molecular Ecology Resources 11(4). p.686-692
Abstract
We present a locus-specific protocol suitable for the investigation, from extracted DNA, into natural inter- and intra-specific genetic variation in a group of nine innate immune genes, all belonging to the β-defensin gene family. The products of these genes encode peptides with antimicrobial properties and form part of the innate immune system. The protocol amplifies the exon coding for the peptide that interacts with invading pathogens and is applicable across a wide range of passerine bird species, although with varying success depending on species. In several species tested, we found individuals to be heterozygous at several of the genes, highlighting the existence of coding genetic variation in this part of the immune system.... (More)
We present a locus-specific protocol suitable for the investigation, from extracted DNA, into natural inter- and intra-specific genetic variation in a group of nine innate immune genes, all belonging to the β-defensin gene family. The products of these genes encode peptides with antimicrobial properties and form part of the innate immune system. The protocol amplifies the exon coding for the peptide that interacts with invading pathogens and is applicable across a wide range of passerine bird species, although with varying success depending on species. In several species tested, we found individuals to be heterozygous at several of the genes, highlighting the existence of coding genetic variation in this part of the immune system. Furthermore, for several of the genes, alleles have been conserved at the amino acid level across taxonomically distant bird species. In one case, we observed the existence of trans-species polymorphisms, often taken as evidence of balancing selection. The method will make it possible to investigate a part of the immune system for which there exists very little information of the genetic variation in wild vertebrate populations, thus making it possible to start investigating the selective forces under which the genes are evolving and the extent to which the found genetic variation is associated with pathogen susceptibility in wild populations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alleleic variation, beta-defensin, birds, innate immunity, trans-species polymorphism
in
Molecular Ecology Resources
volume
11
issue
4
pages
686 - 692
publisher
Wiley-Blackwell
external identifiers
  • wos:000292478500010
  • scopus:79959254891
ISSN
1755-098X
DOI
10.1111/j.1755-0998.2011.02995.x
language
English
LU publication?
yes
id
8110054e-0ede-4765-ad76-9cb6da9e1649 (old id 2028997)
date added to LUP
2011-07-26 11:58:07
date last changed
2017-10-01 03:08:54
@article{8110054e-0ede-4765-ad76-9cb6da9e1649,
  abstract     = {We present a locus-specific protocol suitable for the investigation, from extracted DNA, into natural inter- and intra-specific genetic variation in a group of nine innate immune genes, all belonging to the β-defensin gene family. The products of these genes encode peptides with antimicrobial properties and form part of the innate immune system. The protocol amplifies the exon coding for the peptide that interacts with invading pathogens and is applicable across a wide range of passerine bird species, although with varying success depending on species. In several species tested, we found individuals to be heterozygous at several of the genes, highlighting the existence of coding genetic variation in this part of the immune system. Furthermore, for several of the genes, alleles have been conserved at the amino acid level across taxonomically distant bird species. In one case, we observed the existence of trans-species polymorphisms, often taken as evidence of balancing selection. The method will make it possible to investigate a part of the immune system for which there exists very little information of the genetic variation in wild vertebrate populations, thus making it possible to start investigating the selective forces under which the genes are evolving and the extent to which the found genetic variation is associated with pathogen susceptibility in wild populations.},
  author       = {Hellgren, Olof and Sheldon, B. C.},
  issn         = {1755-098X},
  keyword      = {alleleic variation,beta-defensin,birds,innate immunity,trans-species polymorphism},
  language     = {eng},
  number       = {4},
  pages        = {686--692},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology Resources},
  title        = {Locus-specific protocol for nine different innate immune genes (antimicrobial peptides: beta-defensins) across passerine bird species reveals within-species coding variation and a case of trans-species polymorphisms},
  url          = {http://dx.doi.org/10.1111/j.1755-0998.2011.02995.x},
  volume       = {11},
  year         = {2011},
}