Advanced

Complement activation products in liquid stored plasma and C3a kinetics after transfusion of autologous plasma.

Norda, R; Schött, Ulf LU ; Berséus, O; Åkerblom, O; Nilsson, B; Ekdahl, K N; Stegmayr, B G and Knutson, E F (2012) In Vox Sanguinis 102. p.125-133
Abstract
Background and Objectives Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg) . Material and Methods Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg) , C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors. Results Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg) , C3d,g and sC5b-9 from day 0 and... (More)
Background and Objectives Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg) . Material and Methods Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg) , C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors. Results Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg) , C3d,g and sC5b-9 from day 0 and low iC3, than plasma prepared by other methods. By storage day 7, C3a-(desArg) -levels were above the reference value in 88% of all components. After re-infusion of autologous plasma with high C3a-(desArg) content, there were rapid a(1) and a(2) -distribution followed by a slower b-elimination phase. Conclusion Plasma components prepared by different methods and stored in the liquid phase differ significantly in the amount and timing of complement activation. C3a-(desArg) present in plasma is rapidly eliminated after transfusion. Autologous plasma could be used to study complement kinetics in different clinical situations. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vox Sanguinis
volume
102
pages
125 - 133
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000299206300005
  • pmid:21770955
  • scopus:84856016833
ISSN
1423-0410
DOI
10.1111/j.1423-0410.2011.01522.x
language
English
LU publication?
yes
id
9f286c23-da09-4146-841c-51672b423c33 (old id 2058483)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21770955?dopt=Abstract
date added to LUP
2011-08-01 12:49:37
date last changed
2017-04-23 04:03:29
@article{9f286c23-da09-4146-841c-51672b423c33,
  abstract     = {Background and Objectives Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg) . Material and Methods Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg) , C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors. Results Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg) , C3d,g and sC5b-9 from day 0 and low iC3, than plasma prepared by other methods. By storage day 7, C3a-(desArg) -levels were above the reference value in 88% of all components. After re-infusion of autologous plasma with high C3a-(desArg) content, there were rapid a(1) and a(2) -distribution followed by a slower b-elimination phase. Conclusion Plasma components prepared by different methods and stored in the liquid phase differ significantly in the amount and timing of complement activation. C3a-(desArg) present in plasma is rapidly eliminated after transfusion. Autologous plasma could be used to study complement kinetics in different clinical situations.},
  author       = {Norda, R and Schött, Ulf and Berséus, O and Åkerblom, O and Nilsson, B and Ekdahl, K N and Stegmayr, B G and Knutson, E F},
  issn         = {1423-0410},
  language     = {eng},
  pages        = {125--133},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Vox Sanguinis},
  title        = {Complement activation products in liquid stored plasma and C3a kinetics after transfusion of autologous plasma.},
  url          = {http://dx.doi.org/10.1111/j.1423-0410.2011.01522.x},
  volume       = {102},
  year         = {2012},
}