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Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.

Warfvinge, Karin LU ; Schwartz, Philip H; Kiilgaard, Jens Folke; la Cour, Morten; Young, Michael J; Scherfig, Erik and Klassen, Henry (2011) In Journal of transplantation 2011(Article ID 948740).
Abstract
To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence... (More)
To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of transplantation
volume
2011
issue
Article ID 948740
publisher
Hindawi Publishing Corporation
external identifiers
  • pmid:21766011
ISSN
2090-0015
DOI
10.1155/2011/948740
language
English
LU publication?
yes
id
c283a698-75f4-4c72-8d1e-78d10b565ee4 (old id 2058553)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21766011?dopt=Abstract
date added to LUP
2011-08-01 20:12:05
date last changed
2016-04-15 22:20:00
@article{c283a698-75f4-4c72-8d1e-78d10b565ee4,
  abstract     = {To investigate the feasibility of transplanting human neural progenitor cells (hNPCs) to the retina of nonimmunosuppressed pigs, cultured hNPCs were injected into the subretinal space of 5 adult pigs after laser burns were applied to promote donor cell integration. Postoperatively, the retinal vessels appeared normal without signs of exudation, bleeding, or subretinal elevation. Eyes were harvested at 10-28 days. H&E consistently showed mild retinal vasculitis, depigmentation of the RPE, and marked mononuclear cell infiltrate in the choroid adjacent to the site of transplantation. Human-specific antibodies revealed donor cells in the subretinal space at 10-13 days and smaller numbers within the retina on days 12 and 13, with evidence suggesting a limited degree of morphological integration; however, no cells remained at 4 weeks. The strong mononuclear cell reaction and loss of donor cells indicate that modulation of host immunity is likely necessary for prolonged xenograft survival in this model.},
  articleno    = {948740},
  author       = {Warfvinge, Karin and Schwartz, Philip H and Kiilgaard, Jens Folke and la Cour, Morten and Young, Michael J and Scherfig, Erik and Klassen, Henry},
  issn         = {2090-0015},
  language     = {eng},
  number       = {Article ID 948740},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of transplantation},
  title        = {Xenotransplantation of human neural progenitor cells to the subretinal space of nonimmunosuppressed pigs.},
  url          = {http://dx.doi.org/10.1155/2011/948740},
  volume       = {2011},
  year         = {2011},
}