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Effectiveness of beads for tracking small-scale molecular motor dynamics

Lade, Steven J. ; Craig, Erin M. and Linke, Heiner LU orcid (2011) In Physical Review E (Statistical, Nonlinear, and Soft Matter Physics) 84(2).
Abstract
Investigations into molecular motor dynamics are increasingly focused on small-scale features of the motor's motion. We define performance measures of a common type of single-molecule motility assay, the bead assay, for its ability to detect such features. Using numerical models, we explore the dependence of assay performance on a number of experimentally controllable parameters, including bead size, optical force, and the method of attaching the bead to the motor. We find that the best parameter choice depends on the objective of the experiments, and give a guide to parameter selection. Comparison of the models against experimental data from a recent bead assay of myosin V exemplifies how our methods can also be used to extract additional... (More)
Investigations into molecular motor dynamics are increasingly focused on small-scale features of the motor's motion. We define performance measures of a common type of single-molecule motility assay, the bead assay, for its ability to detect such features. Using numerical models, we explore the dependence of assay performance on a number of experimentally controllable parameters, including bead size, optical force, and the method of attaching the bead to the motor. We find that the best parameter choice depends on the objective of the experiments, and give a guide to parameter selection. Comparison of the models against experimental data from a recent bead assay of myosin V exemplifies how our methods can also be used to extract additional information from bead assays, particularly that related to small-scale features. By analyzing the experimental data we find evidence for previously undetected multiple waiting states of the bead-motor complex. Furthermore, from numerical simulations we find that equilibrium bead dynamics display features previously attributed to aborted motor steps, and that bead dynamics alone can produce multiple subphases during a step. (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Review E (Statistical, Nonlinear, and Soft Matter Physics)
volume
84
issue
2
article number
021907
publisher
American Physical Society
external identifiers
  • wos:000293559200012
  • scopus:80051651129
  • pmid:21929020
ISSN
1539-3755
DOI
10.1103/PhysRevE.84.021907
language
English
LU publication?
yes
id
0fa3d1f0-11ec-4681-bd4a-2a9a4ae894ca (old id 2065419)
date added to LUP
2016-04-01 10:32:15
date last changed
2021-07-27 04:34:59
@article{0fa3d1f0-11ec-4681-bd4a-2a9a4ae894ca,
  abstract     = {Investigations into molecular motor dynamics are increasingly focused on small-scale features of the motor's motion. We define performance measures of a common type of single-molecule motility assay, the bead assay, for its ability to detect such features. Using numerical models, we explore the dependence of assay performance on a number of experimentally controllable parameters, including bead size, optical force, and the method of attaching the bead to the motor. We find that the best parameter choice depends on the objective of the experiments, and give a guide to parameter selection. Comparison of the models against experimental data from a recent bead assay of myosin V exemplifies how our methods can also be used to extract additional information from bead assays, particularly that related to small-scale features. By analyzing the experimental data we find evidence for previously undetected multiple waiting states of the bead-motor complex. Furthermore, from numerical simulations we find that equilibrium bead dynamics display features previously attributed to aborted motor steps, and that bead dynamics alone can produce multiple subphases during a step.},
  author       = {Lade, Steven J. and Craig, Erin M. and Linke, Heiner},
  issn         = {1539-3755},
  language     = {eng},
  number       = {2},
  publisher    = {American Physical Society},
  series       = {Physical Review E (Statistical, Nonlinear, and Soft Matter Physics)},
  title        = {Effectiveness of beads for tracking small-scale molecular motor dynamics},
  url          = {http://dx.doi.org/10.1103/PhysRevE.84.021907},
  doi          = {10.1103/PhysRevE.84.021907},
  volume       = {84},
  year         = {2011},
}