Direct Cannula Implantation in the Cisterna Magna of Pigs
(2021) In Journal of Visualized Experiments 172. p.1-12- Abstract
- The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic... (More)
- The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic system can be studied at a high optical resolution in gyrencephalic brains and in doing so bridges the knowledge gap between rodent and human glymphatics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/206da46f-a092-4945-950b-060c923b7629
- author
- Bechet, Nicholas LU ; Shanbhag, Nagesh C LU and Lundgaard, Iben LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Visualized Experiments
- volume
- 172
- article number
- e62641
- pages
- 1 - 12
- publisher
- JoVE
- external identifiers
-
- pmid:34180907
- scopus:85108412893
- ISSN
- 1940-087X
- DOI
- 10.3791/62641
- language
- English
- LU publication?
- yes
- id
- 206da46f-a092-4945-950b-060c923b7629
- date added to LUP
- 2021-08-19 15:11:58
- date last changed
- 2022-05-12 21:25:30
@article{206da46f-a092-4945-950b-060c923b7629, abstract = {{The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic system can be studied at a high optical resolution in gyrencephalic brains and in doing so bridges the knowledge gap between rodent and human glymphatics.}}, author = {{Bechet, Nicholas and Shanbhag, Nagesh C and Lundgaard, Iben}}, issn = {{1940-087X}}, language = {{eng}}, pages = {{1--12}}, publisher = {{JoVE}}, series = {{Journal of Visualized Experiments}}, title = {{Direct Cannula Implantation in the Cisterna Magna of Pigs}}, url = {{http://dx.doi.org/10.3791/62641}}, doi = {{10.3791/62641}}, volume = {{172}}, year = {{2021}}, }