Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Direct Cannula Implantation in the Cisterna Magna of Pigs

Bechet, Nicholas LU ; Shanbhag, Nagesh C LU and Lundgaard, Iben LU (2021) In Journal of Visualized Experiments 172. p.1-12
Abstract
The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic... (More)
The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic system can be studied at a high optical resolution in gyrencephalic brains and in doing so bridges the knowledge gap between rodent and human glymphatics. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Visualized Experiments
volume
172
article number
e62641
pages
1 - 12
publisher
JoVE
external identifiers
  • pmid:34180907
  • scopus:85108412893
ISSN
1940-087X
DOI
10.3791/62641
language
English
LU publication?
yes
id
206da46f-a092-4945-950b-060c923b7629
date added to LUP
2021-08-19 15:11:58
date last changed
2022-05-12 21:25:30
@article{206da46f-a092-4945-950b-060c923b7629,
  abstract     = {{The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces and has been implicated in the clearance of neurotoxic peptides such as amyloid-beta. Impaired glymphatic function exacerbates disease pathology in animal models of neurodegenerative diseases, such as Alzheimer's, which highlights the importance of understanding this clearance system. The glymphatic system is often studied by cisterna magna cannulations (CMc), where tracers are delivered directly into the cerebrospinal fluid (CSF). Most studies, however, have been carried out in rodents. Here, we demonstrate an adaptation of the CMc technique in pigs. Using CMc in pigs, the glymphatic system can be studied at a high optical resolution in gyrencephalic brains and in doing so bridges the knowledge gap between rodent and human glymphatics.}},
  author       = {{Bechet, Nicholas and Shanbhag, Nagesh C and Lundgaard, Iben}},
  issn         = {{1940-087X}},
  language     = {{eng}},
  pages        = {{1--12}},
  publisher    = {{JoVE}},
  series       = {{Journal of Visualized Experiments}},
  title        = {{Direct Cannula Implantation in the Cisterna Magna of Pigs}},
  url          = {{http://dx.doi.org/10.3791/62641}},
  doi          = {{10.3791/62641}},
  volume       = {{172}},
  year         = {{2021}},
}