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Interaction of Bacteroides fragilis and Bacteroides thetaiotaomicron with the kallikrein-kinin system

Murphy, Elizabeth LU ; Mörgelin, Matthias LU ; Cooney, Jakki C. and Frick, Inga-Maria LU (2011) In Microbiology 157. p.2094-2105
Abstract
Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant... (More)
Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant prolongation of the intrinsic coagulation time and also to the release of bradykinin. Members of the genus Bacteroides have been known to act as opportunistic pathogens outside the gut, with B. fragilis being the most common isolate from clinical infections, such as intra-abdominal abscesses and bacteraemia. The present results thus provide more insight into how Bacteroides species cause infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbiology
volume
157
pages
2094 - 2105
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000293204000021
  • pmid:21527472
  • scopus:79959993374
ISSN
1465-2080
DOI
10.1099/mic.0.046862-0
language
English
LU publication?
yes
id
544a27f2-ed0f-4aff-bb7f-0ddb0529ec57 (old id 2072016)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21527472?dopt=Abstract
date added to LUP
2011-09-02 08:30:23
date last changed
2017-01-01 03:33:03
@article{544a27f2-ed0f-4aff-bb7f-0ddb0529ec57,
  abstract     = {Many bacterial pathogens interfere with the contact system (kallikrein kinin system) in human plasma. Activation of this system has two consequences: cleavage of high-molecular-mass kininogen (HK) resulting in release of the potent proinflammatory peptide bradykinin, and initiation of the intrinsic pathway of coagulation. In this study, two species of the Gram-negative anaerobic commensal organism Bacteroides, namely Bacteroides fragilis and Bacteroides thetaiotaomicron, were found to bind HK and fibrinogen, the major clotting protein, from human plasma as shown by immunoelectron microscopy and Western blot analysis. In addition, these Bacteroides species were capable of activating the contact system at its surface leading to a significant prolongation of the intrinsic coagulation time and also to the release of bradykinin. Members of the genus Bacteroides have been known to act as opportunistic pathogens outside the gut, with B. fragilis being the most common isolate from clinical infections, such as intra-abdominal abscesses and bacteraemia. The present results thus provide more insight into how Bacteroides species cause infection.},
  author       = {Murphy, Elizabeth and Mörgelin, Matthias and Cooney, Jakki C. and Frick, Inga-Maria},
  issn         = {1465-2080},
  language     = {eng},
  pages        = {2094--2105},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology},
  title        = {Interaction of Bacteroides fragilis and Bacteroides thetaiotaomicron with the kallikrein-kinin system},
  url          = {http://dx.doi.org/10.1099/mic.0.046862-0},
  volume       = {157},
  year         = {2011},
}