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A dominant suppressive MHC class II haplotype interacting with autosomal genes controls autoantibody production and chronicity of arthritis

Kutty Selva, Nandakumar LU ; Lindqvist, Anna-Karin LU and Holmdahl, Rikard LU (2011) In Annals of the Rheumatic Diseases 70(9). p.1664-1670
Abstract
Objective To investigate the genetic control of chronic arthritis and collagen epitope specific antibody responses in an experimental model for rheumatoid arthritis. Methods The chronic collagen induced arthritis (CCIA) model was used, induced with collagen type II (CII) in mineral oil lacking mycobacterium in BALB/c (n=24), B10.Q (n=44), (BALB/c x B10.Q) F1 (n=85) and B10.Q x (BALB/c x B10.Q) N2 (n=684) mice. Genome-wide genotyping for 190 N2 mice was performed with extreme phenotypes: chronic arthritis that persisted for 4 months or non-affected. Statistical and linkage analysis were performed with R/qtl software using arthritis and serum subphenotypes. Results (BALB/c x B10. Q) F1 mice were highly prone to develop a chronic relapsing... (More)
Objective To investigate the genetic control of chronic arthritis and collagen epitope specific antibody responses in an experimental model for rheumatoid arthritis. Methods The chronic collagen induced arthritis (CCIA) model was used, induced with collagen type II (CII) in mineral oil lacking mycobacterium in BALB/c (n=24), B10.Q (n=44), (BALB/c x B10.Q) F1 (n=85) and B10.Q x (BALB/c x B10.Q) N2 (n=684) mice. Genome-wide genotyping for 190 N2 mice was performed with extreme phenotypes: chronic arthritis that persisted for 4 months or non-affected. Statistical and linkage analysis were performed with R/qtl software using arthritis and serum subphenotypes. Results (BALB/c x B10. Q) F1 mice were highly prone to develop a chronic relapsing arthritis (66%), whereas both parental strains were relatively resistant: BALB/c (H-2(d); 0%) and B10. Q (H-2(q); 4.5%). CCIA experiments were performed on 684 mice backcrossed to B10. Q; 38% of the mice developed arthritis and more than half of them developed chronic arthritis phenotype. Genome-wide genotyping revealed mainly the major histocompatibility complex (MHC) locus that had an independent and dominant influence on the chronicity. Interestingly, the H2(d) allele had a dominant suppressive effect. This effect overrode the role of other loci as interaction analysis, after conditioning MHC, revealed additional loci, controlling arthritis and autoantibody phenotypes. Conclusions A dominant negative influence of specific MHC haplotype (H2(d)) on CCIA was identified. Further, loci controlling the autoantibody response to different CII epitopes were also identified, and it has been shown that these are dependent on MHC and non-MHC genes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of the Rheumatic Diseases
volume
70
issue
9
pages
1664 - 1670
publisher
British Medical Association
external identifiers
  • wos:000293275600025
  • scopus:80955178845
ISSN
1468-2060
DOI
10.1136/ard.2011.151738
language
English
LU publication?
yes
id
65987555-e44a-46ae-b242-64d017edf7f3 (old id 2072490)
date added to LUP
2011-09-02 08:32:15
date last changed
2017-01-01 06:21:49
@article{65987555-e44a-46ae-b242-64d017edf7f3,
  abstract     = {Objective To investigate the genetic control of chronic arthritis and collagen epitope specific antibody responses in an experimental model for rheumatoid arthritis. Methods The chronic collagen induced arthritis (CCIA) model was used, induced with collagen type II (CII) in mineral oil lacking mycobacterium in BALB/c (n=24), B10.Q (n=44), (BALB/c x B10.Q) F1 (n=85) and B10.Q x (BALB/c x B10.Q) N2 (n=684) mice. Genome-wide genotyping for 190 N2 mice was performed with extreme phenotypes: chronic arthritis that persisted for 4 months or non-affected. Statistical and linkage analysis were performed with R/qtl software using arthritis and serum subphenotypes. Results (BALB/c x B10. Q) F1 mice were highly prone to develop a chronic relapsing arthritis (66%), whereas both parental strains were relatively resistant: BALB/c (H-2(d); 0%) and B10. Q (H-2(q); 4.5%). CCIA experiments were performed on 684 mice backcrossed to B10. Q; 38% of the mice developed arthritis and more than half of them developed chronic arthritis phenotype. Genome-wide genotyping revealed mainly the major histocompatibility complex (MHC) locus that had an independent and dominant influence on the chronicity. Interestingly, the H2(d) allele had a dominant suppressive effect. This effect overrode the role of other loci as interaction analysis, after conditioning MHC, revealed additional loci, controlling arthritis and autoantibody phenotypes. Conclusions A dominant negative influence of specific MHC haplotype (H2(d)) on CCIA was identified. Further, loci controlling the autoantibody response to different CII epitopes were also identified, and it has been shown that these are dependent on MHC and non-MHC genes.},
  author       = {Kutty Selva, Nandakumar and Lindqvist, Anna-Karin and Holmdahl, Rikard},
  issn         = {1468-2060},
  language     = {eng},
  number       = {9},
  pages        = {1664--1670},
  publisher    = {British Medical Association},
  series       = {Annals of the Rheumatic Diseases},
  title        = {A dominant suppressive MHC class II haplotype interacting with autosomal genes controls autoantibody production and chronicity of arthritis},
  url          = {http://dx.doi.org/10.1136/ard.2011.151738},
  volume       = {70},
  year         = {2011},
}