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The YIGI (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae

Granath, K; Modig, Tobias LU ; Forsmark, A; Adler, L and Lidén, Gunnar LU (2005) In Yeast 22(16). p.1257-1268
Abstract
Under anaerobic conditions S. cerevisiae produces glycerol to regenerate NAD+ from the excess NADH produced in cell metabolism. We here report on the role of an uncharacterized protein, Yig1p (YpI201cp), in anaerobic glycerol production. Yig1p was previously shown to interact in two-hybrid tests with the GPP1 and GPP2 encoded glycerol 3-phosphatase (Gpp), and we here demonstrate that strains overexpressing YIG1 show strongly decreased Gpp activity and content of the major phosphatase, Gpp1p. However, cells overexpressing YIG1 exhibited only slightly decreased GPP1 transcript levels, suggesting that Yig1p modulates expression on both transcriptional and post-transcriptional levels. In agreement with such a role, a GFP-tagged derivate of... (More)
Under anaerobic conditions S. cerevisiae produces glycerol to regenerate NAD+ from the excess NADH produced in cell metabolism. We here report on the role of an uncharacterized protein, Yig1p (YpI201cp), in anaerobic glycerol production. Yig1p was previously shown to interact in two-hybrid tests with the GPP1 and GPP2 encoded glycerol 3-phosphatase (Gpp), and we here demonstrate that strains overexpressing YIG1 show strongly decreased Gpp activity and content of the major phosphatase, Gpp1p. However, cells overexpressing YIG1 exhibited only slightly decreased GPP1 transcript levels, suggesting that Yig1p modulates expression on both transcriptional and post-transcriptional levels. In agreement with such a role, a GFP-tagged derivate of Yig1p was localized to both the cytosol and the nucleus. Deletion or overexpression of YIG1 did not, however, significantly affect growth yield or glycerol yield in anaerobic batch cultures, which is consistent with the previously proposed low flux control exerted at the Gpp level. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycerol, phosphatase, GPP1
in
Yeast
volume
22
issue
16
pages
1257 - 1268
publisher
John Wiley & Sons
external identifiers
  • pmid:16358322
  • wos:000234809400001
  • scopus:31144464181
ISSN
1097-0061
DOI
10.1002/yea.1307
language
English
LU publication?
yes
id
f4399b15-de8a-42c4-b2dd-6b0f525f790b (old id 209724)
date added to LUP
2007-09-22 20:48:57
date last changed
2017-01-01 07:16:22
@article{f4399b15-de8a-42c4-b2dd-6b0f525f790b,
  abstract     = {Under anaerobic conditions S. cerevisiae produces glycerol to regenerate NAD+ from the excess NADH produced in cell metabolism. We here report on the role of an uncharacterized protein, Yig1p (YpI201cp), in anaerobic glycerol production. Yig1p was previously shown to interact in two-hybrid tests with the GPP1 and GPP2 encoded glycerol 3-phosphatase (Gpp), and we here demonstrate that strains overexpressing YIG1 show strongly decreased Gpp activity and content of the major phosphatase, Gpp1p. However, cells overexpressing YIG1 exhibited only slightly decreased GPP1 transcript levels, suggesting that Yig1p modulates expression on both transcriptional and post-transcriptional levels. In agreement with such a role, a GFP-tagged derivate of Yig1p was localized to both the cytosol and the nucleus. Deletion or overexpression of YIG1 did not, however, significantly affect growth yield or glycerol yield in anaerobic batch cultures, which is consistent with the previously proposed low flux control exerted at the Gpp level.},
  author       = {Granath, K and Modig, Tobias and Forsmark, A and Adler, L and Lidén, Gunnar},
  issn         = {1097-0061},
  keyword      = {glycerol,phosphatase,GPP1},
  language     = {eng},
  number       = {16},
  pages        = {1257--1268},
  publisher    = {John Wiley & Sons},
  series       = {Yeast},
  title        = {The YIGI (YPL201c) encoded protein is involved in regulating anaerobic glycerol metabolism in Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1002/yea.1307},
  volume       = {22},
  year         = {2005},
}