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The bacterial (Vibrio alginolyticus) production of tetrodotoxin in the ribbon worm Lineus longissimus - Just a false positive?

Strand, Malin; Hedström, Martin LU ; Seth, Henrik; McEvoy, Eric G.; Jacobsson, Erik; Göransson, Ulf; Andersson, Håkan S. and Sundberg, Per (2016) In Marine Drugs 14(4).
Abstract

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and... (More)

We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axonal conductance, LC-MS, Mucus, Nemertean, Tetrodotoxin, Vibrio
in
Marine Drugs
volume
14
issue
4
publisher
Multidisciplinary Digital Publishing Institute (MDPI)
external identifiers
  • Scopus:84962751120
  • WOS:000374588000002
DOI
10.3390/md14040063
language
English
LU publication?
yes
id
20d5b8d3-ff9b-413e-9c11-931d8359ac13
date added to LUP
2016-05-27 12:54:42
date last changed
2017-01-01 08:27:03
@article{20d5b8d3-ff9b-413e-9c11-931d8359ac13,
  abstract     = {<p>We test previous claims that the bacteria Vibrio alginolyticus produces tetrodotoxin (TTX) when living in symbiosis with the nemertean Lineus longissimus by a setup with bacteria cultivation for TTX production. Toxicity experiments on the shore crab, Carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from LC-MS and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male Wistar rat tissue showed conclusively that this effect did not originate from TTX. However, a compound of similar molecular weight was found, albeit apparently non-toxic, and with different LC retention time and MS/MS fragmentation pattern than those of TTX. We conclude that C. maenas paralysis and death likely emanate from a compound </p>},
  articleno    = {63},
  author       = {Strand, Malin and Hedström, Martin and Seth, Henrik and McEvoy, Eric G. and Jacobsson, Erik and Göransson, Ulf and Andersson, Håkan S. and Sundberg, Per},
  keyword      = {Axonal conductance,LC-MS,Mucus,Nemertean,Tetrodotoxin,Vibrio},
  language     = {eng},
  month        = {04},
  number       = {4},
  publisher    = {Multidisciplinary Digital Publishing Institute (MDPI)},
  series       = {Marine Drugs},
  title        = {The bacterial (Vibrio alginolyticus) production of tetrodotoxin in the ribbon worm Lineus longissimus - Just a false positive?},
  url          = {http://dx.doi.org/10.3390/md14040063},
  volume       = {14},
  year         = {2016},
}