Effect of cleaning methods on the dissolution of diatom frustules
Saad, Emily ; Pickering, Rebecca LU
; Shoji, K
; Hossain, M.I.
; Glover, T.G.
; Krause, Jeffrey W.
and Tang, Y
(2020)
In Marine Chemistry
224.
- Abstract
- Experimental studies characterizing the reactivity of siliceous materials, such as biogenic silica from the field or laboratory cultures, typically require organic matter removal prior to experimental analysis. Consequently, it is highly desired to develop and optimize a cleaning protocol that imposes minimal alterations to the reactivity of the siliceous substrate and can be robustly employed among laboratories and investigators. This study offers a quantitative comparison of several methods for removing organic matter associated with biogenic silica to assess their efficacy for use in reactivity studies. Five protocols for organic matter removal were assessed, including combinations of chemical treatments and/or baking, along with low... (More)
- Experimental studies characterizing the reactivity of siliceous materials, such as biogenic silica from the field or laboratory cultures, typically require organic matter removal prior to experimental analysis. Consequently, it is highly desired to develop and optimize a cleaning protocol that imposes minimal alterations to the reactivity of the siliceous substrate and can be robustly employed among laboratories and investigators. This study offers a quantitative comparison of several methods for removing organic matter associated with biogenic silica to assess their efficacy for use in reactivity studies. Five protocols for organic matter removal were assessed, including combinations of chemical treatments and/or baking, along with low temperature ashing with an oxygen plasma. These methods were tested and evaluated using Thalassiosira pseudonana frustules for mass recovery, organic carbon removal, elemental composition, morphology, structural order, relative abundance of silanol (≡Si-OH) groups, and dissolution rate. An additional experiment was conducted over a longer time scale using Thalassiosira weissflogii and a more realistic seawater matrix. Low temperature plasma ashing was found to be the most suitable for organic matter removal in studies seeking to constrain the short-term dissolution of biogenic silica; this method efficiently removed organic carbon while being the least impactful on frustule dissolution compared to the other cleaning methods evaluated. However, if the line of inquiry for an experiment does not require understanding of short-term dissolution rates, commonly used chemical (e.g. mineral acid, peroxide) and high temperature treatments did not appear to affect the long-term trajectory in biogenic silica dissolution. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/20da2ab3-742a-4d35-a156-e1dc1e69bd8e
- author
- Saad, Emily
; Pickering, Rebecca
LU
; Shoji, K
; Hossain, M.I.
; Glover, T.G.
; Krause, Jeffrey W.
and Tang, Y
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Diatoms, Biogenic silica, Thalassiosira, Dissolution, Sediment cleaning
- in
- Marine Chemistry
- volume
- 224
- article number
- 103826
- publisher
- Elsevier
- external identifiers
-
- scopus:85085341047
- ISSN
- 1872-7581
- DOI
- 10.1016/j.marchem.2020.103826
- language
- English
- LU publication?
- no
- id
- 20da2ab3-742a-4d35-a156-e1dc1e69bd8e
- date added to LUP
- 2023-05-18 14:45:22
- date last changed
- 2023-05-28 17:58:54
@article{20da2ab3-742a-4d35-a156-e1dc1e69bd8e,
abstract = {{Experimental studies characterizing the reactivity of siliceous materials, such as biogenic silica from the field or laboratory cultures, typically require organic matter removal prior to experimental analysis. Consequently, it is highly desired to develop and optimize a cleaning protocol that imposes minimal alterations to the reactivity of the siliceous substrate and can be robustly employed among laboratories and investigators. This study offers a quantitative comparison of several methods for removing organic matter associated with biogenic silica to assess their efficacy for use in reactivity studies. Five protocols for organic matter removal were assessed, including combinations of chemical treatments and/or baking, along with low temperature ashing with an oxygen plasma. These methods were tested and evaluated using Thalassiosira pseudonana frustules for mass recovery, organic carbon removal, elemental composition, morphology, structural order, relative abundance of silanol (≡Si-OH) groups, and dissolution rate. An additional experiment was conducted over a longer time scale using Thalassiosira weissflogii and a more realistic seawater matrix. Low temperature plasma ashing was found to be the most suitable for organic matter removal in studies seeking to constrain the short-term dissolution of biogenic silica; this method efficiently removed organic carbon while being the least impactful on frustule dissolution compared to the other cleaning methods evaluated. However, if the line of inquiry for an experiment does not require understanding of short-term dissolution rates, commonly used chemical (e.g. mineral acid, peroxide) and high temperature treatments did not appear to affect the long-term trajectory in biogenic silica dissolution.}},
author = {{Saad, Emily and Pickering, Rebecca and Shoji, K and Hossain, M.I. and Glover, T.G. and Krause, Jeffrey W. and Tang, Y}},
issn = {{1872-7581}},
keywords = {{Diatoms; Biogenic silica; Thalassiosira; Dissolution; Sediment cleaning}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Marine Chemistry}},
title = {{Effect of cleaning methods on the dissolution of diatom frustules}},
url = {{http://dx.doi.org/10.1016/j.marchem.2020.103826}},
doi = {{10.1016/j.marchem.2020.103826}},
volume = {{224}},
year = {{2020}},
}