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Retinal neurospheres prepared as tissue for transplantation

Liljekvist Soltic, Ingela LU and Johansson, K (2005) In Developmental Brain Research 160(2). p.194-202
Abstract
The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and leukemia inhibitory factor (LIF). Neurospheres labeled with a lipophilic dye were placed onto explants, and tissue interactions were analyzed after 2-6 days of culture. Immunocytochemical analysis of neurospheres revealed the presence of neuronal and glial cells. Proliferating neuronal and glial cells were observed after 2... (More)
The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and leukemia inhibitory factor (LIF). Neurospheres labeled with a lipophilic dye were placed onto explants, and tissue interactions were analyzed after 2-6 days of culture. Immunocytochemical analysis of neurospheres revealed the presence of neuronal and glial cells. Proliferating neuronal and glial cells were observed after 2 weeks, whereas the neuronal cell proliferation declined considerably after 4 weeks. Few apoptotic cells were observed in the neurospheres. Neurospheres cultured on explanted adult retina engrafted with the surrounding tissue, but progenitor cell migration into the explants was low. However, the grafted neurospheres appeared to limit the experimentally induced photoreceptor apoptosis in the surrounding explant tissue. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
photoreceptor, progenitor cell, retina, transplant, neuroprotection
in
Developmental Brain Research
volume
160
issue
2
pages
194 - 202
publisher
Elsevier
external identifiers
  • wos:000234161000007
  • pmid:16290209
  • scopus:28444452928
ISSN
0165-3806
DOI
10.1016/j.devbrainres.2005.09.003
language
English
LU publication?
yes
id
4e9e5750-6c9c-45ae-82ed-58bceef0b6f2 (old id 210570)
date added to LUP
2007-08-23 16:49:02
date last changed
2017-01-01 07:23:11
@article{4e9e5750-6c9c-45ae-82ed-58bceef0b6f2,
  abstract     = {The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and leukemia inhibitory factor (LIF). Neurospheres labeled with a lipophilic dye were placed onto explants, and tissue interactions were analyzed after 2-6 days of culture. Immunocytochemical analysis of neurospheres revealed the presence of neuronal and glial cells. Proliferating neuronal and glial cells were observed after 2 weeks, whereas the neuronal cell proliferation declined considerably after 4 weeks. Few apoptotic cells were observed in the neurospheres. Neurospheres cultured on explanted adult retina engrafted with the surrounding tissue, but progenitor cell migration into the explants was low. However, the grafted neurospheres appeared to limit the experimentally induced photoreceptor apoptosis in the surrounding explant tissue.},
  author       = {Liljekvist Soltic, Ingela and Johansson, K},
  issn         = {0165-3806},
  keyword      = {photoreceptor,progenitor cell,retina,transplant,neuroprotection},
  language     = {eng},
  number       = {2},
  pages        = {194--202},
  publisher    = {Elsevier},
  series       = {Developmental Brain Research},
  title        = {Retinal neurospheres prepared as tissue for transplantation},
  url          = {http://dx.doi.org/10.1016/j.devbrainres.2005.09.003},
  volume       = {160},
  year         = {2005},
}