Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies
(2005) In Journal of Neuroscience Methods 149(2). p.101-109- Abstract
- Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron... (More)
- Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron microscopic studies. We demonstrate also that this method can be combined with post-embedding immunogold labeling to detect amino acid transmitters in synaptic boutons. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/211128
- author
- Havton, LA and Broman, Jonas LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- intraperitoneal, visceral, GABA, immunogold
- in
- Journal of Neuroscience Methods
- volume
- 149
- issue
- 2
- pages
- 101 - 109
- publisher
- Elsevier
- external identifiers
-
- wos:000233831400002
- pmid:16054225
- scopus:27844463520
- ISSN
- 1872-678X
- DOI
- 10.1016/j.jneumeth.2005.03.014
- language
- English
- LU publication?
- yes
- id
- 0f66abd9-cfe5-4083-82c3-bdce1245e562 (old id 211128)
- date added to LUP
- 2016-04-01 12:23:16
- date last changed
- 2022-04-21 06:43:21
@article{0f66abd9-cfe5-4083-82c3-bdce1245e562,
abstract = {{Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron microscopic studies. We demonstrate also that this method can be combined with post-embedding immunogold labeling to detect amino acid transmitters in synaptic boutons.}},
author = {{Havton, LA and Broman, Jonas}},
issn = {{1872-678X}},
keywords = {{intraperitoneal; visceral; GABA; immunogold}},
language = {{eng}},
number = {{2}},
pages = {{101--109}},
publisher = {{Elsevier}},
series = {{Journal of Neuroscience Methods}},
title = {{Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies}},
url = {{http://dx.doi.org/10.1016/j.jneumeth.2005.03.014}},
doi = {{10.1016/j.jneumeth.2005.03.014}},
volume = {{149}},
year = {{2005}},
}