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Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies

Havton, LA and Broman, Jonas LU (2005) In Journal of Neuroscience Methods 149(2). p.101-109
Abstract
Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron... (More)
Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron microscopic studies. We demonstrate also that this method can be combined with post-embedding immunogold labeling to detect amino acid transmitters in synaptic boutons. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intraperitoneal, visceral, GABA, immunogold
in
Journal of Neuroscience Methods
volume
149
issue
2
pages
101 - 109
publisher
Elsevier
external identifiers
  • wos:000233831400002
  • pmid:16054225
  • scopus:27844463520
ISSN
1872-678X
DOI
10.1016/j.jneumeth.2005.03.014
language
English
LU publication?
yes
id
0f66abd9-cfe5-4083-82c3-bdce1245e562 (old id 211128)
date added to LUP
2007-08-24 13:55:39
date last changed
2017-01-01 05:05:58
@article{0f66abd9-cfe5-4083-82c3-bdce1245e562,
  abstract     = {Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing for labeling of neuronal populations that are difficult to label with local tracer injections. Here, we demonstrate in the adult rat that intraperitoneal administration of cholera toxin subunit B conjugated to horseradish peroxidase labels preganglionic autonomic neurons, motoneurons, and the terminal projections of select primary afferents for both light and electron microscopic studies. We demonstrate also that this method can be combined with post-embedding immunogold labeling to detect amino acid transmitters in synaptic boutons.},
  author       = {Havton, LA and Broman, Jonas},
  issn         = {1872-678X},
  keyword      = {intraperitoneal,visceral,GABA,immunogold},
  language     = {eng},
  number       = {2},
  pages        = {101--109},
  publisher    = {Elsevier},
  series       = {Journal of Neuroscience Methods},
  title        = {Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies},
  url          = {http://dx.doi.org/10.1016/j.jneumeth.2005.03.014},
  volume       = {149},
  year         = {2005},
}