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Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo

Pera, Edgar LU ; Hou, Shirui LU ; Strate, Ina LU ; Wessely, O and De Robertis, EM (2005) In International Journal of Developmental Biology 49(7). p.781-796
Abstract
Secreted proteins playa crucial role in intercellular communication during embryogenesis and in the adult. We recently described a novel method, designated as secretion cloning, that allows identifying extracellular proteins exclusively based on their ability to be secreted by transfected cells. In this paper, we present the results of a large-scale screening of more than 90,000 clones from three cDNA expression libraries constructed from early Xenopus embryos. Of 170 sequenced clones, 65 appeared to encode secreted proteins; 26 clones (40%) were identical to previously known Xenopus genes, 25 clones (38%) were homologous to other genes identified in various organisms and 14 clones (22%) were novel. Apart from these bona fide secreted... (More)
Secreted proteins playa crucial role in intercellular communication during embryogenesis and in the adult. We recently described a novel method, designated as secretion cloning, that allows identifying extracellular proteins exclusively based on their ability to be secreted by transfected cells. In this paper, we present the results of a large-scale screening of more than 90,000 clones from three cDNA expression libraries constructed from early Xenopus embryos. Of 170 sequenced clones, 65 appeared to encode secreted proteins; 26 clones (40%) were identical to previously known Xenopus genes, 25 clones (38%) were homologous to other genes identified in various organisms and 14 clones (22%) were novel. Apart from these bona fide secreted proteins, we also isolated lysosomal or other secretory pathway proteins and some cytoplasmic proteins commonly found in body fluids. Among the novel secreted proteins were two putative growth factors of the Granulin family, termed xGra1 and xGra2; they are structurally similar to EGF and TGF alpha and show a spotted expression pattern in the epidermis. Another secreted protein, designated xSOUL, belongs to the family of heme-binding proteins and exhibits distinct expression in the early brain. Athird protein, termed Xystatin, is related to cysteine proteinase inhibitors. Our results indicate that secretion cloning is an effective and generally useful tool for the unbiased isolation of secreted proteins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
secretion cloning, Spemann-Mangold organizer, gastrula, lysosomal, maternal
in
International Journal of Developmental Biology
volume
49
issue
7
pages
781 - 796
publisher
U B C Press
external identifiers
  • pmid:16172975
  • wos:000233799200001
  • scopus:27144545798
ISSN
1696-3547
DOI
10.1387/ijdb.052003ep
language
English
LU publication?
yes
id
353b2cf0-cce5-4787-bc7e-12479049832a (old id 211152)
date added to LUP
2007-08-10 16:17:48
date last changed
2017-01-15 03:42:46
@article{353b2cf0-cce5-4787-bc7e-12479049832a,
  abstract     = {Secreted proteins playa crucial role in intercellular communication during embryogenesis and in the adult. We recently described a novel method, designated as secretion cloning, that allows identifying extracellular proteins exclusively based on their ability to be secreted by transfected cells. In this paper, we present the results of a large-scale screening of more than 90,000 clones from three cDNA expression libraries constructed from early Xenopus embryos. Of 170 sequenced clones, 65 appeared to encode secreted proteins; 26 clones (40%) were identical to previously known Xenopus genes, 25 clones (38%) were homologous to other genes identified in various organisms and 14 clones (22%) were novel. Apart from these bona fide secreted proteins, we also isolated lysosomal or other secretory pathway proteins and some cytoplasmic proteins commonly found in body fluids. Among the novel secreted proteins were two putative growth factors of the Granulin family, termed xGra1 and xGra2; they are structurally similar to EGF and TGF alpha and show a spotted expression pattern in the epidermis. Another secreted protein, designated xSOUL, belongs to the family of heme-binding proteins and exhibits distinct expression in the early brain. Athird protein, termed Xystatin, is related to cysteine proteinase inhibitors. Our results indicate that secretion cloning is an effective and generally useful tool for the unbiased isolation of secreted proteins.},
  author       = {Pera, Edgar and Hou, Shirui and Strate, Ina and Wessely, O and De Robertis, EM},
  issn         = {1696-3547},
  keyword      = {secretion cloning,Spemann-Mangold organizer,gastrula,lysosomal,maternal},
  language     = {eng},
  number       = {7},
  pages        = {781--796},
  publisher    = {U B C Press},
  series       = {International Journal of Developmental Biology},
  title        = {Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo},
  url          = {http://dx.doi.org/10.1387/ijdb.052003ep},
  volume       = {49},
  year         = {2005},
}