Advanced

Exposure to PCB and p,p '-DDE in European and Inuit populations: impact on human sperm chromatin integrity

Spano, M; Toft, G; Hagmar, L; Eleuteri, P; Rescia, M; Rignell-Hydbom, Anna LU ; Tyrkiel, E; Zvyezday, V and Bonde, JP (2005) In Human Reproduction 20(12). p.3488-3499
Abstract
BACKGROUND: Persistent organochlorine pollutants ( POP), such as polychlorinated biphenyls (PCB) and dichlorodiphenyldichloroethylene (p,p'-DDE), are widely found in the environment and considered potential endocrine-disrupting compounds (EDC). Their impact on male fertility is still unknown. METHODS: To explore the hypothesis that POP is associated with altered sperm chromatin integrity, a cross-sectional study involving 707 adult males ( 193 Inuits from Greenland, 178 Swedish fishermen, 141 men from Warsaw, Poland, and 195 men from Kharkiv, Ukraine) was carried out. Serum levels of 2,2', 4,4', 5,5'-hexachlorobiphenyl (CB-153), as a proxy of the total PCB burden, and of p,p'-DDE were determined. Sperm chromatin structure assay (SCSA) was... (More)
BACKGROUND: Persistent organochlorine pollutants ( POP), such as polychlorinated biphenyls (PCB) and dichlorodiphenyldichloroethylene (p,p'-DDE), are widely found in the environment and considered potential endocrine-disrupting compounds (EDC). Their impact on male fertility is still unknown. METHODS: To explore the hypothesis that POP is associated with altered sperm chromatin integrity, a cross-sectional study involving 707 adult males ( 193 Inuits from Greenland, 178 Swedish fishermen, 141 men from Warsaw, Poland, and 195 men from Kharkiv, Ukraine) was carried out. Serum levels of 2,2', 4,4', 5,5'-hexachlorobiphenyl (CB-153), as a proxy of the total PCB burden, and of p,p'-DDE were determined. Sperm chromatin structure assay (SCSA) was used to assess sperm DNA/chromatin integrity. RESULTS: We found a strong and monotonically increasing DNA fragmentation index with increasing serum levels of CB-153 among European but not Inuit men, reaching a 60% higher average level in the highest exposure group. No significant associations were found between SCSA-derived parameters and p,p'-DDE serum concentrations. CONCLUSION: These results suggest that human dietary PCB exposure might have a negative impact on the sperm chromatin integrity of adult males but additional issues, including differences in the genetic background and lifestyle habits, still need to be elucidated. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sperm chromatin, SCSA, polychlorinated biphenyls (PCB), DDT, DNA damage
in
Human Reproduction
volume
20
issue
12
pages
3488 - 3499
publisher
Oxford University Press
external identifiers
  • wos:000233668700034
  • pmid:16223788
  • scopus:28544438093
ISSN
0268-1161
DOI
10.1093/humrep/dei297
language
English
LU publication?
yes
id
0ab72f5d-8f0b-4404-83e0-e9acefe86809 (old id 211620)
date added to LUP
2007-08-13 09:36:11
date last changed
2017-10-29 03:30:41
@article{0ab72f5d-8f0b-4404-83e0-e9acefe86809,
  abstract     = {BACKGROUND: Persistent organochlorine pollutants ( POP), such as polychlorinated biphenyls (PCB) and dichlorodiphenyldichloroethylene (p,p'-DDE), are widely found in the environment and considered potential endocrine-disrupting compounds (EDC). Their impact on male fertility is still unknown. METHODS: To explore the hypothesis that POP is associated with altered sperm chromatin integrity, a cross-sectional study involving 707 adult males ( 193 Inuits from Greenland, 178 Swedish fishermen, 141 men from Warsaw, Poland, and 195 men from Kharkiv, Ukraine) was carried out. Serum levels of 2,2', 4,4', 5,5'-hexachlorobiphenyl (CB-153), as a proxy of the total PCB burden, and of p,p'-DDE were determined. Sperm chromatin structure assay (SCSA) was used to assess sperm DNA/chromatin integrity. RESULTS: We found a strong and monotonically increasing DNA fragmentation index with increasing serum levels of CB-153 among European but not Inuit men, reaching a 60% higher average level in the highest exposure group. No significant associations were found between SCSA-derived parameters and p,p'-DDE serum concentrations. CONCLUSION: These results suggest that human dietary PCB exposure might have a negative impact on the sperm chromatin integrity of adult males but additional issues, including differences in the genetic background and lifestyle habits, still need to be elucidated.},
  author       = {Spano, M and Toft, G and Hagmar, L and Eleuteri, P and Rescia, M and Rignell-Hydbom, Anna and Tyrkiel, E and Zvyezday, V and Bonde, JP},
  issn         = {0268-1161},
  keyword      = {sperm chromatin,SCSA,polychlorinated biphenyls (PCB),DDT,DNA damage},
  language     = {eng},
  number       = {12},
  pages        = {3488--3499},
  publisher    = {Oxford University Press},
  series       = {Human Reproduction},
  title        = {Exposure to PCB and p,p '-DDE in European and Inuit populations: impact on human sperm chromatin integrity},
  url          = {http://dx.doi.org/10.1093/humrep/dei297},
  volume       = {20},
  year         = {2005},
}