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Cytogenetic methods

Gisselsson, David LU (2015) In Cancer Cytogenetics p.11-18
Abstract

This chapter outlines the methods currently employed in cancer cytogenetics, spanning from chromosome banding to array- and sequencing-based techniques. A correct sampling procedure is the basis for correct scientific and diagnostic conclusions. Chromosome preparation requires live cells, whereas in situ hybridization at least requires intact nuclei, and genome arrays as well as sequencing rely on DNA that has not been extensively degraded. Direct preparations or short-term cultures are therefore usually preferred for chromosome banding analysis. In situ hybridization techniques are based on the inherent organization of DNA into two antiparallel complementary strands. Genomic arrays are highly efficient tools for obtaining data on... (More)

This chapter outlines the methods currently employed in cancer cytogenetics, spanning from chromosome banding to array- and sequencing-based techniques. A correct sampling procedure is the basis for correct scientific and diagnostic conclusions. Chromosome preparation requires live cells, whereas in situ hybridization at least requires intact nuclei, and genome arrays as well as sequencing rely on DNA that has not been extensively degraded. Direct preparations or short-term cultures are therefore usually preferred for chromosome banding analysis. In situ hybridization techniques are based on the inherent organization of DNA into two antiparallel complementary strands. Genomic arrays are highly efficient tools for obtaining data on genomic imbalances present in a tumor sample. The advent of massive parallel/second-generation/ next-generation sequencing (NGS technology has radically transformed the field of cancer cytogenetics. More than 800 genomes of more than 25 cancer types have now been sequenced.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Cancer cytogenetics, Chromosome banding, Chromosome preparation, Genomic arrays, In situ hybridization techniques, Next-generation sequencing
in
Cancer Cytogenetics
editor
Heim, Sverre ; Mitelman, Felix; and
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85015907051
ISBN
9781118795538
9781118795569
DOI
10.1002/9781118795569.ch2
language
English
LU publication?
yes
id
211caca9-f3d0-4e80-9798-d73efcc99bd5
date added to LUP
2017-04-20 10:33:12
date last changed
2017-04-20 10:33:12
@inbook{211caca9-f3d0-4e80-9798-d73efcc99bd5,
  abstract     = {<p>This chapter outlines the methods currently employed in cancer cytogenetics, spanning from chromosome banding to array- and sequencing-based techniques. A correct sampling procedure is the basis for correct scientific and diagnostic conclusions. Chromosome preparation requires live cells, whereas in situ hybridization at least requires intact nuclei, and genome arrays as well as sequencing rely on DNA that has not been extensively degraded. Direct preparations or short-term cultures are therefore usually preferred for chromosome banding analysis. In situ hybridization techniques are based on the inherent organization of DNA into two antiparallel complementary strands. Genomic arrays are highly efficient tools for obtaining data on genomic imbalances present in a tumor sample. The advent of massive parallel/second-generation/ next-generation sequencing (NGS technology has radically transformed the field of cancer cytogenetics. More than 800 genomes of more than 25 cancer types have now been sequenced.</p>},
  author       = {Gisselsson, David},
  editor       = {Heim, Sverre  and Mitelman, Felix},
  isbn         = {9781118795538},
  keyword      = {Cancer cytogenetics,Chromosome banding,Chromosome preparation,Genomic arrays,In situ hybridization techniques,Next-generation sequencing},
  language     = {eng},
  pages        = {11--18},
  publisher    = {Wiley-Blackwell},
  series       = {Cancer Cytogenetics},
  title        = {Cytogenetic methods},
  url          = {http://dx.doi.org/10.1002/9781118795569.ch2},
  year         = {2015},
}